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High yield lactic acid production using mixed cultures

Active Publication Date: 2022-06-30
NATURES PRINCIPLES BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is about a method and apparatus for increasing the yield of lactic acid production by specifically addressing the impact of proteins and B vitamins in the feedstock. The method involves a cycle of feeding and replenishing the reactor with a specific volume of feed and effluent, based on the pH levels. The apparatus includes a cheap reactor and containers for feed, effluent, and replenishment, along with a controller for managing the pH levels and feed additions. Overall, the invention provides a more efficient and effective way of producing lactic acid.

Problems solved by technology

The downside is that somewhat lower yields (70% versus 90%) are obtained, which may be due to more biomass and possible byproduct, and in general there is no direct control over D / L ratio lactic acid; however, under specific conditions the latter can be resolved.
And most importantly, none of the references demonstrate an “adaptive cycling” process.

Method used

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  • High yield lactic acid production using mixed cultures
  • High yield lactic acid production using mixed cultures

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[0045]Materials and Methods

[0046]Reactor Operation

[0047]The enrichment was carried out in a 3 L bioreactor with a working volume of 2 L. Anaerobic conditions were maintained by continuously sparging the reactor with N2 at a rate of 216 mL min-1 (@T=273K, P=105 kPa). The culture was taken out of the reactor for biofilm removal from reactor walls and head three times per week. The reactor was continuously agitated at a speed of 300 rpm using mechanical stirrers. Reactor temperature was maintained at 30□C by recirculating water heated at 31° C. (E300 thermostat, Lauda, Germany) in the outer wall of the reactor. To prevent culture broth evaporation, the off-gas was cooled using a cryostat set to 5° C. Reactor pH was maintained at 5.0±0.2 using 8 mol L−1 NaOH and 1 mol L−1 HCl solutions (ADI 1030 Bio controller, Applikon, The Netherlands). To prevent excessive foaming, anti-foaming agent (3% v:v anti-foam C, Sigma Aldrich, Germany) was added in equal amounts and at equal speed as NaOH du...

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Abstract

The present invention is in the field of a method of producing lactic acid in high yield in a sequencing batch reactor. Therein glucose may be used as feedstock for bacteria, which ferment the glucose into lactic acid. The reactor is operated under at least partly defined non-axenic conditions and in a cyclic mode.

Description

FIELD OF THE INVENTION[0001]The present invention is in the field of a method of producing lactic acid in high yield in a sequencing batch reactor. Therein glucose or other sugars may be used as feedstock for bacteria, which ferment the glucose into lactic acid. The reactor is at least partly operated under defined conditions and in a cyclic mode.BACKGROUND OF THE INVENTION[0002]Lactic acid (CH3CH(OH)CO2H) is a simple organic chemical compound that can be used in many applications. It is a chiral molecule and it occurs as L- or D-lactic acid. It is noted that lactic acid is highly soluble.[0003]Lactic acid fermentation is performed on an industrial scale by lactic acid bacteria (Lactobacillus species), which convert simple carbohydrates such as glucose, sucrose, or galactose to lactic acid, or by chemical synthesis. Lactic acid producing bacteria can produce two moles of lactate from one mole of glucose, or one mole of lactate from one mole of glucose as well as carbon dioxide and a...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12M1/34C12M1/02C12M1/00C12N1/20
CPCC12P7/56C12M41/26C12N1/20C12M29/18C12M41/22C12M23/58C12M41/36
Inventor ROMBOUTS, JULIUS LAURENSVAN LOOSDRECHT, MARINUS CORNELIUS MARIAKLEEREBEZEM, ROBBERTWEISSBRODT, DAVID GREGORYSTOUTEN, GERBENALLAART, MAXIMILIENNE TOETIE
Owner NATURES PRINCIPLES BV