Methods, strains, and compositions useful for microbially enhanced oil recovery: Arcobacter clade 1

a technology of microorganisms and oil recovery, applied in the direction of bacteria based processes, sealing/packing, borehole/well accessories, etc., can solve the problems of poor sweep efficiency, poor sweep efficiency, and inability to recover oil in the less permeable zone, so as to achieve the effect of enhancing oil recovery

Inactive Publication Date: 2016-06-28
EI DU PONT DE NEMOURS & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]wherein growth of the Arcobacter in the oil reservoir enhances oil recovery.

Problems solved by technology

One problem commonly encountered with water flooding operations is poor sweep efficiency of injection water.
Poor sweep efficiency occurs when water preferentially channels through highly permeable zones of the oil reservoir as it travels from the injection well(s) to the production well(s), thus bypassing less permeable oil-bearing strata.
Oil in the less permeable zones is thus not recovered.
Poor sweep efficiency may also be due to differences in the mobility of the water versus that of the oil.

Method used

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  • Methods, strains, and compositions useful for microbially enhanced oil recovery: <i>Arcobacter </i>clade 1
  • Methods, strains, and compositions useful for microbially enhanced oil recovery: <i>Arcobacter </i>clade 1
  • Methods, strains, and compositions useful for microbially enhanced oil recovery: <i>Arcobacter </i>clade 1

Examples

Experimental program
Comparison scheme
Effect test

example 1

Anaerobic Enrichment for Indigenous Microbes from Oil Reservoir Samples

[0155]To enrich for species that could reduce any of the electron acceptors nitrate, fumarate or the ferric ion (Fe (III), we inoculated 1 mL of either injection water or production water from Well #2, described in General Methods, into 9 mL of minimal salts media (Table 4) in 20 mL anaerobic serum vials, supplemented with lactate (2000 ppm) as the carbon source, sodium chloride to 4300 ppm and as electron acceptor either 1.6 g / L sodium nitrate, or 3.5 g / L sodium fumarate, or 13,000 ppm NaEDTAFe(III). A fourth enrichment having a rich medium, Marine broth (Difco™ B. D. Diagnostics Sparks Md.), which was supplemented with sodium chloride to 3900 ppm and lactate to 2000 ppt, was used to enrich for microbes that require more than a minimal medium for growth. The electron acceptor in the Marine broth sample was scavenged from the Fe (III), sulfate, nitrate and organic molecules in the formulation that could be used a...

example 2

Characterization of Isolated Strains with Respect to Arcobacter sp.

[0161]To determine the 16S rDNA sequence of the seven isolates named 97AE 3-12, 97AE3-1, 97AE 3-3, 97AE 3-7, 97AE 4-6, 97AE 4-5, and 97AE4-1 (Example 1), each of the seven isolates was picked as a pure single colony, DNA was isolated and the 16S rRNA gene was amplified by PCR using the procedures in General Methods. The amplified sequences were cloned into pCR-TOPO4 vector using the TOPO TA cloning system (Invitrogen), as recommended by the manufacturer, and then sequenced multiple times using primers 1492R, 8F, M13 Reverse, and M13 Forward of SEQ ID NOs:43-46, respectively, to obtain the near full sequence. Each strain 16S rDNA sequence (97AE 3-12: SEQ ID NO:1, 97AE3-1: SEQ ID NO:33, 97AE 3-3: SEQ ID NO:34, 97AE 3-7: SEQ ID NO:35, 97AE 4-6: SEQ ID NO:38, 97AE 4-5: SEQ ID NO:37, and 97AE4-1: SEQ ID NO:36) was queried against the NCBI (National Center for Biotechnology Information) database using the BLAST (Basic Loca...

example 3

Riboprinting to Determine Strain Differences

[0186]The Arcobacter sp. strains isolated in Example 1: 97AE3-12, 97AE3-1, 97AE 3-3, 97AE 3-7, 97AE 4-6, 97AE 4-5, and 97AE4-1 were subjected to automated Riboprinter® analysis, as described in General Methods, to determine whether these isolated strains were unique with respect to one another. As a reference strain, Arcobacter halophilus (ATCC strain BAA-1022), which has 16S rDNA with 96.4% sequence identity to strain 97AE3-12, was included in the analysis. While all of these strains have significant sequence identity to one another in the 16S rDNA, several unique rDNA RiboPrint™ patterns were obtained. As shown in FIG. 4, the patterns of EcoRI restriction fragments which hybridized to 16S and 23S rDNA probes were different for Arcobacter sp. representative strains 97AE3-12 (ATCC #PTA-11409), 97AE3-3 (ATCC #PTA-11410), and 97AE3-1. This analysis showed that the genomic sequences surrounding the 16S and 23 rRNA genes in these strains are d...

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Abstract

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to Arcobacter clade 1 and medium including an electron acceptor. The Arcobacter strains grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery.

Description

[0001]This application claims the benefit of U.S. Provisional Application 61 / 408,739, filed Nov. 1, 2010 and is incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]This disclosure relates to the field of environmental microbiology and modification of crude oil well properties using microorganisms. More specifically, methods for improving oil recovery from an underground reservoir are presented and new microorganisms are identified that can be used for oil recovery.BACKGROUND OF THE INVENTION[0003]During recovery of oil from oil reservoirs, typically only a minor portion of the original oil in the oil-bearing strata is recovered by primary recovery methods which use only the natural forces present in an oil reservoir. To improve oil recovery, a variety of supplemental recovery techniques such as water flooding, which involves injection of water through well bores into the oil reservoir, have been used. As water moves into the reservoir from an injection well and mov...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): C09K8/58C09K8/582C12N1/20C12R1/01
CPCC09K8/582C12N1/20C12R1/01C12N1/205C12R2001/01
Inventor HENDRICKSON, EDWIN R.JACKSON, SCOTT CHRISTOPHERLUCKRING, ABIGAIL K
Owner EI DU PONT DE NEMOURS & CO
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