The invention relates to a method and a device for measuring optical system lateral magnification by utilizing uniform-speed moving point target, belonging to the field of metering equipment taking an optical method as a characteristic. In the method, a points target is imaged under the uniform-speed motion state, so that a linear image is obtained; and in a frequency domain, a value range of pitch of pixels is found, and because the actual modification transfer function curve relative to a pixel pitch and a theoretical modification transfer function curve have the best contact ratio under the least square condition, and the optical system lateral magnification is calculated by a search algorithm to be obtained. In the device, a sliding block for bearing the point target is arranged on first rails and second rails, a controller controls the sliding block to do uniform-speed motion on the first rails, and at the same time, controls the sliding block to do motion on the second rails, and the motions in the two directions are matched, so that during the motion process, the point target is always focused to be imaged onto the surface of an image senor. By adopting the method and the device to measure the optical system lateral magnification rate, the errors between single-time measurement results can be reduced, and further, the repeatability of the measurement results can be improved.