The invention provides a fruit fresh-keeping box which can keep fruit fresh for a long time and is very convenient to assemble, disassemble and take. The fruit fresh-keeping box comprises a box body and multiple layers of fruit trays arranged in a containing cavity of the box body, the box body comprises a sealing cover and a box main body, the box main body is provided with a thermal-insulation layer and a containing layer, the thermal-insulation layer and the containing layer are separated, a hollow interlayer is arranged between the thermal-insulation layer and the containing layer, the containing layer is fixedly connected with the thermal-insulation layer through a connection portion, and the hollow interlayer is filled with polyving akohol and borax coolants; sliding rail sets arranged layer by layer at intervals are arranged on the inner wall of the containing layer, each fruit tray is provided with two sets of pulleys, and the pulleys are embedded in the sliding rail sets; the box body is provided with an air pump, a pressure gauge, a direct-current power supply and a controller capable of automatically starting and shutting off the air pump according to pressure values read from the pressure gauge, and the box body is further provided with a preservative container and a spraying device. The fruit fresh-keeping box has the advantages that fresh-keeping time is long and can reach more than three months, the fruit bearing portions of the fruit trays are reasonable in structure, convenient to take and put and convenient to clean and can stand stably.