The present invention relates to preparation process of Yang strengthening, fatigue removing and calcium supplementing oral liquor. The Yang strengthening, fatigue removing and calcium supplementing oral liquor is prepared with superfine crushed micron ginseng powder, micron antler powder, micron pine flower powder, micron pearl powder, oligose powder and distilled water, and through combination and processing. It has obvious Yang strengthening effect, fatigue removing effect and calcium supplementing effect as well as the functions of strengthening immunity, tranquilizing, strengthening eyesight, etc. It can provide middle aged people and old people with rich protein, vitamins, minerals, active enzymes and other nutritious matters, to strengthen health.