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Process for preparing semen momordicae seed extract containing triterpene saponin component

The invention relates to a technology of Momordicae semen and extract, which is applied in the field of preparation of extracts containing triterpene saponins from traditional Chinese medicine Momordicae semen, and can solve the problems that adjuvants cannot be directly applied to Momordicae semen.

Inactive Publication Date: 2007-09-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to develop the application value of traditional Chinese medicine in my country, there are currently researches on the application of triterpenoid saponin extracts from the fruit of Sapia chinensis in immune adjuvants. The poisonous semen japonicus mainly used for external use cannot be directly used as an adjuvant. The purpose of the present invention is to prepare an extract containing active ingredients of triterpene saponins in the semen semen japonicus, remove its toxic components, and make the application of the semen semen arborii safe and effective. reliable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 30 kilograms of wood turtle seeds (purchased from the Chinese herbal medicine market in Haozhou, Anhui Province), were extracted in 3 times, 10 kilograms each time. First, the seeds of the wood turtle were crushed and soaked in 40-50% ethanol for 24 hours; then, the ethanol was refluxed for 3 times, each time for 2 hours; the extract was filtered, and the filtrate was concentrated under reduced pressure (R502B rotary evaporator was purchased from Shanghai Shensheng Technology Co., Ltd.); 101 clarifier (Shanghai Watson Biological Engineering Co., Ltd.) was added to the concentrated solution to make the final concentration 5‰ (101 clarifier to concentrated solution weight-to-volume ratio), fully mixed, heated to 60°C in a water bath, and statically Set aside for 24 hours; take the supernatant, extract 3 times with ether to remove fat-soluble components; add water-saturated n-butanol to extract 4 times to remove insoluble components in n-butanol, concentrate under reduced p...

Embodiment 2

[0018] The seed kernel and the hard seed coat of the fruit of the wood turtle were separated to obtain 0.597 kg of the seed kernel and 0.401 kg of the hard seed coat. The seed kernel and hard seed coat were extracted respectively according to the above method. Obtain 0.45g from 0.401kg hard exocarp, the yield is 1.12g / kg; obtain 2.02g from 0.597kg seed kernel, the yield is 3.38g / kg.

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Abstract

The preparation process of semen momordicae extract containing triterpene saponin includes crushing semen momordicae, alcohol reflux extraction, ethyl ether extraction to eliminate liposulable component, n-butanol extraction to eliminate n-butanol insoluble component, and purification with adsorptive macroporous resin to obtain yellowish scented semen momordicae extract powder. Physical and chemical analysis and hemolytic experiment show that the semen momordicae extract contains triterpene saponin component. The semen momordicae extract has low toxicity, may be used in orally taking and injection safely and has wide application foreground in vaccine preparing field.

Description

technical field [0001] The invention belongs to the extraction technology of effective components of traditional Chinese medicine, and relates to a preparation method of the extract of triterpene saponins from the fruit of the traditional Chinese medicine carapace. Background technique [0002] Semen momordicae is the dried and mature seed of Momordica Cochinchinesis (Lour.) Spreng., a plant of Cucurbitaceae. Wood softshell turtle is a perennial vine, mainly distributed in Southeast Asian countries, Mongolia, and most parts of southern my country, such as Guangxi, Sichuan, Hubei, Hunan, Guizhou, Yunnan, Guangdong, Anhui and other provinces. Generally, the mature fruit is harvested in winter, cut open, sun-dried to half-dry, remove the pulp, take out the seeds, and dry in the sun for later use. Mu Biezi is a commonly used traditional Chinese medicine, bitter in the mouth, slightly sweet, cool in nature, has the functions of dispelling stagnation, reducing swelling, attacking...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/42C07J73/00C07G3/00
Inventor 胡松华萧琛闻
Owner ZHEJIANG UNIV