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Method for cultivating cathaya argyrophylla mycorrhiza seedling

A technology for mycorrhizal seedlings and silver fir, which is applied in the field of cultivating silver fir mycorrhizal seedlings, can solve the problems of affecting plant growth, excessive bacterial growth, etc., and achieves the effects of high induction efficiency, simple operation and suitable for popularization and application.

Inactive Publication Date: 2008-02-20
INST OF BOTANY CHINESE ACAD OF SCI +1
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Problems solved by technology

The main technical obstacle is the inability to effectively induce the simultaneous growth of plant roots and fungi, often resulting in excessive growth of fungi, thereby affecting plant growth

Method used

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  • Method for cultivating cathaya argyrophylla mycorrhiza seedling
  • Method for cultivating cathaya argyrophylla mycorrhiza seedling

Examples

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Embodiment 1

[0021] Embodiment 1, the cultivation of silver fir mycorrhizal seedlings

[0022] 1. Germination induction of silver fir seeds

[0023] After 4 weeks of low-temperature treatment at 4 degrees for 4 weeks, the silver fir seeds collected in Dayao Mountain, Guangxi were gently scrubbed with lotion, and then rinsed with running water for several hours. Disinfect the surface with 70% alcohol for 30 seconds, soak in 30% hydrogen peroxide solution for 30 minutes, and then transfer to sterile filter paper to dry. Finally, culture on agar medium containing 0.5% glucose. The temperature and light conditions of the culture room are 23±2° C., 16 hours of light, and the light intensity is 4000 lx. After culturing for 3 days, cut the endosperm of the seed with a scalpel in the ultra-clean workbench, preferably 1 / 3-1 / 5 of the periphery of the endosperm of the seed, and then culture it on the same medium. In 10-20 days, the radicle and germ of Silver Fir began to germinate. After the abov...

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Abstract

The present invention discloses one fast culture method of mycorrhizal silver fir seedling. The fast mycorrhizal silver fir seedling culturing process includes the steps of culturing small silver fir seedling of 2-6 cm height; and planting the small silver fir seedling in composed culture medium and inoculating C. geophilum as mycorrhizal fungus in the culture medium to culture mycorrhizal silver fir seedling. The method of the present invention has high inducing efficiency and simple operation, and is suitable for wide application.

Description

technical field [0001] The invention relates to a method for cultivating silver fir mycorrhizal seedlings. Background technique [0002] Silver fir (Cathaya argyrophylla Chun et Kuang) is a monotypic genus of Pinaceae discovered in my country in the 1950s. It is a rare and endangered plant unique to my country and is known as the "giant panda among plants". [0003] Silver fir is mainly distributed in the subtropical mountainous areas of my country. At present, it is only distributed in a small amount in Guangxi, Sichuan, Guizhou, Hunan and other places in my country. The distribution area is narrow, the adaptability to the ecological environment is poor, and the classification system is relatively isolated. Under natural conditions, silver fir reproduces by seeds, but the amount of fruiting of silver fir is very small, and the germination rate is not high, so the natural reproductive ability of silver fir is low. At the same time, the survival rate is very low when propag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01H17/00A01C1/00
Inventor 傅禄敏李玉花邢树堂谢宗强伦志明
Owner INST OF BOTANY CHINESE ACAD OF SCI
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