Quick separation method for rice leaf spot bacteria

Abstract

This is a method for fast separation of ripe white leaf blight germ, and it is belonged to microbe technology sphere, comporing of such steps: sterilize the target pathogenic bacteria-containing ripe fault tissue with splash for 60-90 seconds; flush it with asepsis water; put the fault tissue on asepsis glass slide and drip asepsis water; put at room temperature for 5-15min; when the pathogenic bacteria puffs from the fault tissue to the water arrouding its notch, use mycetome-diping shift-cymidone to line at NA or SX culture nedium, which suit to grow the ripe white leaf blight germ; culture at 25-28deg C and the corresponding target bacteria is achieved. To separated bacteria according this invention has these advantages: rapid, nicety, pollution rate is low, purity is high and strain varies few, and this holds the wildness characteristic of pathogenic bacteria.

Description

Technical field: [0001] The invention belongs to the technical field of microorganisms, and relates to a rapid separation method for rice bacterial blight bacteria. Background technique: [0002] Rice bacterial blight is one of the most important bacterial diseases of rice production in Asia and the Pacific. Rice bacterial blight is a major rice disease caused by pathogenic bacteria (Xanthomonas oryzae pv.oryzae). It not only seriously occurs in Southeast Asian countries, but also has spread to Australia, America and Africa. At present, in my country except Xinjiang, it has occurred to varying degrees in the whole country, and it is the second most important disease of rice blast only. After the rice is damaged, the yield is generally reduced by 20% to 30%, and in severe cases, the yield is reduced by 50% or even no grains are harvested. The cultivation and utilization of rice disease-resistant varieties and the epidemiological research of diseases, as well as the race dif...

AI Technical Summary

Problems solved by technology

The diseased tissue is broken and diluted and plated to separate the method. The main mechanism of this method is to crush the diseased tissue first, extract an appropriate amount of bacteria-containing liquid with sterile water, and then after doubling dilution, place the diluted liquid on an appropriate medium for separation and culture. The pretreatment time of the above two methods is long, the former is as high as 20 hours, and the latter takes 5 hours to separate a sample; the operation takes up a lot of space, uses consumables and consumes a lot of samples, and in addition crushes diseased tissues, causing a large number of endogenous bacteria or saprophytes Bacterial immersion extracts diseased tissues, resulting in a high pollution rate. The above method is not suitable for the rapid isolation of rice bacterial blight. Low, high pollution level, often due to untimely treatment of diseased tissue samples, making it difficult to process samples and wasting a lot of specimens

The purpose of rapid isolation of ideal pathogens in experiments, selection of isolates, and pathogenicity requirements cannot be achieved

Images