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Fetid aspergillic strain and uses

A technology of Aspergillus stifidum and Aspergillus stifidum, which is applied in the field of high-yielding α-galactosidase strains, can solve the problem of low enzyme-producing level, lack of systematic research on the fermentation characteristics, nutritional characteristics and fermentation costs of enzyme-producing bacteria, and restrictions on soybean milk. product application and other issues, to achieve the effect of reducing production costs, easy application and high stability

Inactive Publication Date: 2008-09-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] At present, microorganisms are also used to produce α-galactosidase, but the existing α-galactosidase producing bacteria are mostly solid-state fermentation, which limits the application in soybean milk products, and the enzyme production level is generally low, which is harmful to enzyme production. The fermentation characteristics, nutritional characteristics and fermentation costs of bacteria have not been systematically studied

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0012] 1. Isolation and identification of strains

[0013] 1.1 strain screening

[0014] From fermented soybean products: tempeh, stinky tofu, fermented bean curd, soy sauce fermented grains (collected and fermented for 4 days, 12 days and 60 days respectively) and rotten soybean meal (soaked in water and fermented for 5 days), as well as yeast strains preserved in the laboratory, and less spore roots Mold, Penicillium point, Aspergillus niger, Aspergillus oryzae, and Bacillus natto were screened and used as samples. The fermented soybean products are enriched and cultured with the proliferation medium, and a single colony is picked on the primary screening medium. After cultivating for an appropriate time, the colony showing blue color on the primary screening medium is picked out for re-screening. A strain with high enzyme-producing activity was obtained by re-screening the fermentation and numbered G5, and the enzyme activity reached 22.27U / mL after being cultured at 28°C ...

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Abstract

This invention discloses Aspergillus foetidus strain that is preserved at China General Microbiological Culture Collection Center (CGMCC) with the name of Aspergillus foetidus ZU-G1. The preservation date is March 3th, 2006, and the preservation number is CGMCC No.1628. the strain can be used to manufacture alpha-galactosidase.

Description

technical field [0001] The invention relates to a bacterial strain with high alpha-galactosidase production, in particular to an Aspergillus foetidus strain. Background technique [0002] α-galactosidase is an oligosaccharide characterized by the specific hydrolysis of non-reducing terminal α-1,6-galactosidic linkages of galacto-oligosaccharides and polygalacto-(gluco)mannan Hydrolase has great economic value and application value. In recent years, α-galactosidase is mainly used in industries such as medicine, feed and food. α-Galactosidase is mainly used to hydrolyze oligosaccharides such as melibiose, raffinose, stachyose and verbascose in beans and polysaccharides of galactomannan to improve sugar metabolism. [0003] Soybean will be an ideal food substitute under the situation of population increase and food shortage. However, the fly in the ointment is that the oligosaccharides contained in soybeans such as melibiose, raffinose, stachyose and a small amount of verbasc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/40C12R1/66
Inventor 何国庆刘彩琴阮晖陈启和李龙
Owner ZHEJIANG UNIV
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