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Culture medium and culture method for cotton immature embryo in vitro culture and plantlet formation

A technology of in vitro culture and culture medium, applied in the fields of botanical equipment and methods, horticultural methods, plant cells, etc., can solve the problems of low adult plant rate, difficulty in germination of immature embryos, and long incubation time of immature embryos.

Inactive Publication Date: 2008-10-22
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many culture media and methods for in vitro culture of cotton immature embryos, there are still problems such as long incubation time of immature embryos, difficulty in germination of immature embryos, and low adult plant rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The cotton varieties tested were Zhongwu 642 and Han 93-2. The young bolls of two varieties 20-25 days after flowering were picked and dried for 3 days in a culture room at a temperature of 28±2° C. and a relative humidity of 40% to 50%. Soak in 75% ethanol for about 30 seconds and then quickly ignite to sterilize the surface of the cotton boll. Use a scalpel to cut the cotton boll along the gap on the surface of the cotton boll, take out the young cotton seeds and peel the young embryos, and transfer the cotton young embryos to the sterilized CEM medium (L-proline 20mg / L, L- Glutamine 500mg / L, indolebutyric acid 0.1mg / L, 6-benzylaminopurine 0.1mg / L), and then the culture flask for culturing immature embryos was sealed with a sealing film and cultured in a culture room. The temperature of the incubation room is 28±2°C, the light intensity is 1500~2500lux, the relative humidity is 40%~50%, 14h light / 10h dark. After 10-15 days of culturing, statistics on cotton embryos and se...

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PUM

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Abstract

The invention discloses a culture medium and a culture method for the in vitro culture and plant formation of young cotton embryos. Based on cross design and gradient experiment. The macroelements in the culture medium are half of those in the MS medium; the trace elements are the same as those in the MS medium; the organic components and the dosage are the same as those in the B5 medium; proline, glutamine, etc. and plant formation beneficial nutrients; with a certain amount of phytohormones and young cotton boll drying and other cultivation methods, the direct germination and plant formation of cotton isolated immature embryos are realized. Using this culture system to cultivate isolated cotton immature embryos 20-25 days after flowering can cause the immature embryos to germinate directly and develop into plants, and the seedling rate of the immature embryos can reach more than 40%.

Description

Technical field [0001] The invention relates to a plant tissue culture method and a culture medium, in particular to a special culture medium for in vitro culture and plant formation of cotton immature embryos and a culture method thereof. Background technique [0002] Cotton belongs to Malvaceae (Malvaceae) and Gossypium (Gossypium). In addition to 4 commonly used cultivated species, there are more than 30 wild species. The wild and related species of the cotton genus contain many excellent genes for salt resistance, drought resistance, and disease and insect resistance. The transfer of these beneficial genes to cultivated species through interspecific hybridization is an effective way to broaden the genetic basis of cotton. However, due to genetic isolation between species, hybrid embryos are often aborted, making it difficult to cross successfully. The in vitro culture technology of immature embryos (beads) artificially improves the development conditions of immature embryos, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 马峙英王彦霞王省芬赵晶张桂寅
Owner HEBEI AGRICULTURAL UNIV.