Micro cell hollow fiber reactor

Abstract

The micro hollow fiber cell reactor consists of silica gel tube of permeable silica gel material, permeable silica gel stopper and hollow fiber filament. Cell is embedded inside the hollow fiber filament with collagen gel, and the micro hollow fiber cell reactor has oxygen transferring and mass transferring capacity suitable for cell to survive and maintains the medicine metabolizing and other functions of cell. The micro hollow fiber cell reactor combines the advantages of miniature orifice plate and 3D bionic artificial liver reactor, maintains the bioactivity of animal cell. It may be used as in vitro mold of liver cell for the research of medicine metabolism and pharmacological and toxicological research and may be used in the lab research of cell culturing condition and cell product. It may be also used as the carrier for the research of animal cell culturing condition and lays the foundation for industrial production.

Description

technical field

[0001] The invention relates to the use of a miniature cell hollow fiber reactor. Background technique

[0002] The existing drug pharmacology and toxicology studies are based on animal models, in vitro primary cell models and continuous cell line models. Animal models have disadvantages such as long experimental period, low efficiency, large individual differences leading to large deviations in results, which are not conducive to animal protection, and cannot eliminate species differences, which is the fatal weakness of animal models. Although the continuous cell line has a simple culture method, high efficiency and low cost, there are still large differences in structure and function between the continuous cell line and the primary cultured cells, and cannot fully reflect the metabolism and toxicity of drugs in humans or animals.

[0003] At present, researchers at home and abroad have begun to pay attention to the application of in vitro primary cell mode...

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