Amolops loloensis insulin release promoting peptide and use in producing medicine

A technology of insulin and turbulent frog, which is applied in the field of biomedicine, can solve problems such as large side effects, failure to cover metabolic defects, failure of pancreatic β cells, etc., and achieve the effect of promoting insulin release

Inactive Publication Date: 2008-12-24
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Claims
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Problems solved by technology

However, due to the large side effects of sulfonylurea drugs, long-term use will lead to the failure of pancreatic β cells due to excessive secretion of insulin. Among diabetics taking such drugs, 10% of patients will switch to insulin every year due to treatment failure. other drugs
Moreover, current pharmacological research and treatment methods do not yet cover all the metabolic defects involved in the disease

Method used

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  • Amolops loloensis insulin release promoting peptide and use in producing medicine

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Embodiment Construction

[0012] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0013] Preparation of insulin release-promoting peptide from brown-spotted frog:

[0014] Clean the living brown-spotted turbulent frog with water, put it in a glass container with a cover, add anhydrous ether (1 liter of volume plus 1 ml of anhydrous ether), and seal the container for 3-5 minutes, and a large amount of foamy substance can be seen It is secreted from the skin of the brown-spotted frog, and the secretion is collected, centrifuged (10000rpm, 20 minutes), freeze-dried and stored.

[0015] In the first step, Sephadex G-50 gel filtration column chromatography, 300 mg of lyophilized powder of brown-spotted frog skin secretion obtained by the above method was dissolved in 5 ml of purified water, and centrifuged at 10,000 rpm at 4°C for 10 min to remove the precipitate. Sephadex G-50 gel filtration column (...

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Abstract

The present invention belongs to the field of biomedicine technology, and is especially Amolops loloensis insulin release promoting peptide and its application in preparing medicine. The present invention obtains insulin release promoting peptide from the skin secretion of Amolops loloensis through a conventional biochemical process, measures its sequence and synthesizes the polypeptide based on the obtained sequence. Amolops loloensis insulin release promoting peptide is one kind of single strand polypeptide of molecular weight of 1627.04 dalton, isoelectri point of 8.75 and total sequence of Phe-Leu-Pro-Ile-Val-Gly-Lys-Leu-Leu-Ser-Gly-Leu-Ser-Gly-Leu- Leu(FLPIVGKLLSGLSGLL-NH2). It has excellent insulin release promoting effect, no hemolytic activity, no plasma clotting activity and other advantages, and may be used in preparing medicine for treating diabetes.

Description

Technical field: [0001] The invention provides an insulin release-promoting peptide of the brown-spotted frog and its application in pharmacy, belonging to the technical field of biomedicine. Background technique: [0002] Diabetes mellitus is a multiple endocrine and metabolic disease caused by multiple genetic and environmental factors with sugar, fat, and protein metabolism disorders, resulting in multi-system and multi-organ dysfunction. Statistics show that in my country in the 1980s, it was <1%, and it has risen to 3.6-5% at present, of which more than 90% (about 38 million people) belong to type II diabetes. As of 2000, there were 135 million people with diabetes in the world, and it is expected to increase to 299 million in 2025. Diabetes itself is not terrible, what is terrible is the complications, which include cancer, sleep apnea, depression, myocardial infarction, peripheral vascular disease, hypertension and the most common type II diabetes. Type II diabet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P3/10
Inventor 赖仞梁建国李宗杰徐春花李东升吕毅
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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