Full-automatic polypeptide synthetic instrument

Abstract

This invention involves a full-automatic peptide synthesizer, including a CPU system, a GMP software operating system, a liquid control system, a gas control system, a materials input system, a reactor system, a limitless speed-regulating stirring system, a personal safety system, a reaction efficiency automatic feedback system, a fault alarm system, and a solution circulatory system. The invention has the advantages of higher synthesis efficiency of peptide, better product quality, shorter development cycles, lower production cost, and more convenient and safe operation. The invention is also consistent with the strict requirements of FDA on GMP production, meets a variety of synthetic peptide research and production requirements on the whole, and can be widely applied to basic research, peptide drugs, health, beauty, food, polymer materials, fine chemicals, organic synthesis, etc.

Description

technical field [0001] The invention relates to an automatic polypeptide synthesizer. Background technique [0002] Since Dr. Bruce Merrifield successfully synthesized the first polypeptide by inventing the revolutionary solid-phase synthesis method in 1962, it has become possible for scientists to perform complex organic reactions in an automated manner. In 1985, the world's first peptide synthesizer was successfully developed, which can shorten the time required for peptide synthesis from one month to one or two days, greatly improve the synthesis efficiency, and also make the development of peptide science advance by leaps and bounds. [0003] At present, there are more than 20 kinds of peptide drugs approved for sale in the market, and the annual sales have already exceeded 10 billion U.S. dollars, and are increasing at a rate of 20% every year. In addition, more than 40 peptide drugs have been approved for clinical trials. It is estimated that at least 3-5 new peptide ...

AI Technical Summary

Problems solved by technology

Some major common shortcomings of these synthesizers include: first, the operating system is outdated, and some of them are still DOS systems, and the synthesis records can be changed at will, which does not meet the certification requirements of the US FDA on GMP production; second, it cannot be realized The linear amplification function from small batch to large batch prolongs the product trial production cycle; thirdly, due to the design defect of the stirrer other than ABI433, the reaction is prone to dead ends, so that the synthesis cannot be completed; with the extension of the peptide chain, The net result of this cumulative negative effect will be a rapid decline in crude yield and purity

For long peptides with more than 15 peptides and peptides with low condensation rate, the negative effect is particularly obvious, and it is difficult to obtain qualified products; fourth, there is no reagent recycling function, which increases the cost and increases the treatment capacity of synthetic waste liquid ; Fifth, the pipeline design is unreasonable, which leads to the mixing of different types of reagents, resulting in cross-contamination and affecting the quality of synthesis; sixth, there is no personal safety protection device, which may cause injury to operators in an emergency

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