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Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein

A technology of human serum protein and serum protein, which is applied in the field of chiral induction to shorten the reaction time and improve the action rate.

Inactive Publication Date: 2009-07-01
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the existing technique of using a template method to induce the optical activity of achiral cyanine dye aggregates, which can only be induced to produce cyanine dye aggregates with a single optical activity, and is limited by the induced template and the structure of the dye. Deficiency of inability to controllably induce the desired chiral aggregates, thus providing a simple and efficient method for chiral induction of achiral cyanine dye aggregates using human serum albumin, which allows the control of aggregate molecules The chiral inversion of , to obtain the desired optical activity of chiral aggregates

Method used

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  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein
  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein
  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein

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Experimental program
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Effect test

Embodiment 1

[0025] Add 0.5ml 80.0μM phosphate buffer solution (pH 8.0) of achiral cyanine dye aggregates in a 10.00ml volumetric flask, then add 0.4ml 50.0μM phosphate buffer solution (pH 8.0) of human serum albumin, the mixed solution The molar concentration ratio of human serum protein to achiral cyanine dye aggregates is 0.5:1; then dilute the mixture with phosphate buffer solution until the concentration of achiral cyanine dye aggregates is 5 μM; shake the mixture well, avoid After 12 hours of light reaction, the induced cyanine dye aggregates were obtained, which were analyzed by UV-visible absorption spectrum and circular dichroism, as shown in figure 2 As shown, the absorption peak (686nm) of the dye aggregate on the absorption spectrum decreases, and the characteristic peak (615nm) of the dye monomer appears in the short-wave direction, and the signal of the aggregate appears on the circular dichroism spectrum, indicating that the dye aggregate is in the human serum albumin. Phot...

Embodiment 2

[0029] Add 0.5ml 80.0μM phosphate buffer solution (pH 6.0) of achiral cyanine dye aggregates in a 10.00ml volumetric flask, then add 1.0ml 50.0μM phosphate buffer solution (pH 6.0) of human serum albumin, the mixture in The molar concentration ratio of human serum protein to achiral cyanine dye aggregates is 1.125:1; then dilute the mixture with phosphate buffer solution until the concentration of achiral cyanine dye aggregates is 5 μM; shake the mixture well, avoid After 9 hours of light reaction, the induced cyanine dye aggregates were obtained. After UV-Vis absorption spectrum and circular dichroism analysis, the absorption peak (686nm) of the dye aggregates in the absorption spectrum decreased, and the characteristic peak of the dye monomer appeared in the short wave direction ( 615nm), the signals of aggregates appear on the circular dichroism spectrum, indicating that the dye aggregates produce photoactivity under the action of human serum albumin. According to the peak s...

Embodiment 3

[0031] Add 0.5ml 80.0μM phosphate buffer solution (pH 7.0) of achiral cyanine dye aggregates in a 10.00ml volumetric flask, then add 1.6ml 50.0μM phosphate buffer solution (pH 7.0) of human serum albumin, the mixture in The molar concentration ratio of human serum protein to achiral cyanine dye aggregates is 2:1; then dilute the mixture with phosphate buffer solution until the concentration of achiral cyanine dye aggregates is 5 μM; shake the mixture well, avoid After 15 hours of photoreaction, the induced cyanine dye aggregates were obtained, which were analyzed by UV-visible absorption spectrum and circular dichroism, as shown in image 3 As shown, the absorption peak (686nm) of the dye aggregate on the absorption spectrum is significantly reduced, and the characteristic peak (615nm) of the stronger dye monomer appears in the short wave direction, and the signal of the aggregate appears on the circular dichroism spectrum, indicating that the dye aggregate is in the Photoacti...

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Abstract

The invention relates to a method for chiral induction of achiral cyanine dye aggregates by using human serum protein, the method comprising: respectively dissolving the achiral cyanine dye and human serum protein aggregates in phosphate buffer solution to prepare the concentration 80.0μM and 50.0μM solutions; mix the above two solutions so that the molar concentration ratio of human serum albumin and achiral cyanine dye aggregates in the mixture is 0.5-5:1; then mix the mixture with phosphate buffer solution The concentration of the achiral cyanine dye aggregate was diluted to 5 μM; the mixed solution was protected from light for 9 to 15 hours to obtain the induced cyanine dye aggregate, which was analyzed by ultraviolet-visible absorption spectrum and circular dichroism. Chirality and chirality inversion. The method can very effectively change the achiral dye aggregate solution into a chiral solution; and the optical rotation of the induced dye aggregate solution can be adjusted by regulating the amount of added human serum albumin.

Description

technical field [0001] The invention relates to a method for chiral induction of non-chiral cyanine dye aggregates by using human serum albumin. Background technique [0002] The chemical name of the cyanine dye is 3,3'-disulfopropyl-4,5,4',5'-dibenzo-9-phenyl-thiacarbocyanine dye-triethylamine salt (C 43 h 47 N 3 o 6 S 4 ), its structural formula is as follows: [0003] [0004] It has long been discovered that cyanine dyes have unique light-sensitive properties, and they are used as developers, photosensitizers, nonlinear optical materials, etc. Recently, it has been discovered that cyanine dyes also have the effect of inhibiting cancer cells, and can be used to prepare drugs for treating cancer. Whether it is used as an optical material in photoresponsive devices or as a drug, specific optical isomers are required. Usually, in aqueous solution, cyanine dye molecules self-assemble to form aggregates through non-covalent bond forces. Aggregates assembled from ach...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B23/04C09B67/10C07K14/765
Inventor 张亚周杜鸿雁向俊峰唐亚林徐广智
Owner INST OF CHEM CHINESE ACAD OF SCI