Quick detection method for estimating capability of culture medium for separating and recycling microbe groups
A detection method, separation and recovery technology, which is applied in the field of rapid detection to evaluate the ability of culture medium to separate and recover microbial groups, can solve the problems of heavy workload, easy misjudgment, time-consuming, etc., and achieve high repeatability
Inactive Publication Date: 2009-07-01
GUANGDONG INST OF MICROORGANISM
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Problems solved by technology
However, because the morphology of microbial colonies is relatively simple, and some are similar, it cannot be judged accurately by microscope alone, and misjudgment is prone to occur, so colony counting based on morphological analysis alone is inaccurate
However, it is almost impossible to classify and identify cultured microorganisms based on their physiological and biochemical characteristics due to heavy workload and time-consuming
Therefore, the traditional method of evaluating the ability of the medium to separate and recover microbial groups has great drawbacks.
Method used
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Embodiment 1
[0017] A rapid detection kit for evaluating the ability of medium to separate and recover microbial groups: including 10 times buffer, dNTP (10mM), F341-357 (GC) (10μM), R534-518 (10μM), Taq (5U / μl) and ddH 2 O.
[0018] This test kit is used to evaluate the ability of different culture media to separate and recover microbial groups:
[0019] (1) Select environmental samples for the experiment: the soil was collected from the Dafeng Experimental Base of the Guangdong Academy of Agricultural Sciences. During the tomato fruiting period, tomato plants were selected according to the five-point sampling method, and the roots were dug out together with the soil, air-dried naturally, and ground Pass through a 1mm sieve;
[0020] (2) Isolation and purification of bacteria in environmental samples: using nutrient broth, YG (yeast dextrose agar medium), root exudates, and soil immersion liquid 4 kinds of medium; fully shake the soil sample, prepare 10 -1 ~10 -5 Samples with differen...
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Abstract
The present invention relates to a kind of using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), according to DGGE electrophoresis pattern and band similarity coefficient (Cs) analysis, comprehensive evaluation different culture medium or culture condition reclaims and separates microbial group capacity . The present invention can intuitively screen out the culture medium that can better separate and recover the microbial groups in the sample, and compared with the traditional method of identification using morphology and physiological and biochemical characteristics, it has the advantages of rapidity, simplicity, accuracy, and high repeatability. The assay can also be used to rapidly analyze changes in environmental microbial population diversity and community dynamics.
Description
[Affiliated technical field] [0001] The invention relates to a rapid detection method for evaluating the ability of recovering and separating microbial groups under different culture media or culture conditions by using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DENATURINGGRADIENT GEL ELECTROPHORESIS, abbreviated as PCR-DGGE). [Background technique] [0002] The microbial resources in the natural environment are extremely rich, but so far, the microorganisms that can be cultured artificially are extremely limited, less than 1% of the total. Due to the lack of culture conditions and culture methods, many bacteria that are dominant in the environment have not been isolated in the laboratory so far, or the isolated bacteria are not the dominant bacteria in the environment at all. It is therefore extremely important to evaluate the ability of different media and culture conditions to recover isolated microbial taxa. [0003] Whether the isolated micr...
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IPC IPC(8): G01N27/447C12Q1/02G01N1/28
Inventor 朱红惠孙晓棠姚青龙良鲲
Owner GUANGDONG INST OF MICROORGANISM