Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetic composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement
A technology for cosmetic compositions and extracts, which can be used in medical preparations, cosmetics, and drug combinations containing active ingredients, and can solve problems such as uncertain effects, high prices, and unstable chemical mixtures.
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Embodiment 1
[0124] Example 1: Extracting active ingredients from Chinese Bauhinia
[0125] Preliminary separation of antioxidant active fractions
[0126] In order to extract active ingredients with antioxidant activity from Chinese Bauhinia, according to the figure 1 Experiments are performed on the process presented in the schematic. Specifically, 1 Kg of leaves and stems of Bauhinia sinensis overcast was crushed into a powder using a pulverizer, and the powder was extracted twice with ethanol (EtOH) at room temperature with two weeks between the two times. To this extract, 0 to 100% ethanol was gradually added thereto (10% increments each time) to prepare an ethanol solution. The prepared ethanol solution was used for extraction. By using DPPH (1,1-diphenyl-2-Pycryl-hydrazine) (Taco, T. et al., Biosci.Biotech.Biochem., 1994,58,1780-1783; Na, M.K. et al., Nat.Prod .Sci., 2002, 8, 26-29) method to study the antioxidant activity of the extract. DPPH is a stable free radical that ...
Embodiment 2
[0167] Embodiment 2: the test of scavenging DPPH free radical activity
[0168] In order to study the antioxidant activity of the extracts isolated in the above-mentioned Example 1 and containing the compounds represented by -, their DPPH free radical scavenging activity was measured by the same method used in the above-mentioned Example 1 . At this time, BHA (tert-butyl-4-hydroxyanisole) and α-tocopherol, a synthetic antioxidant, were used as positive controls.
[0169] As a result, phenolic acids and flavonoids showed dose-dependent strong free radical scavenging activity. However, 1,2-stilbene compounds showed strong free radical scavenging activity. In particular, gallic acid esters including gallic acid have strong activity. For example, the IC of a compound represented by 50 The values are 5.1±0.4, 5.3±0.3, 7.0±1.1, 6.8±0.5, 6.7±0.4 and 8.6±0.7g / ml: (gallic acid), (methyl gallate), (ethyl gallate), ((-)-epicatechin (epicatechin)-3-O-gallate), ((-)-epicatech...
Embodiment 3
[0173] Example 3: Test of Inhibitory Activity of Lipid Peroxidation
[0174] In order to measure the antioxidant activity of the compounds isolated in Example 1 above and containing the compounds represented by to , the lipid peroxidation inhibitory activity of these compounds was investigated. Lipid oxidation produces oxides such as lipid hydroperoxides, conjugated fatty acids (HODEs), epoxy fatty acids, propionaldehyde (MDA) and 4-hydroxynonenal (4-HNE), thus leading to Direct damage to the biofilms that make up cells or indirect reactions with other cellular components. Therefore, lipid oxidation is considered to be one of the main causes of degenerative diseases and aging (Esterbauer, H. et al., FreeRadic.Biol.Med., 1991, 11: 81-128; Esterbauer, H., Cheeseman, K.H., Methods Enzymol ., 1990, 186: 407-421; Jira, W., et al., Chem. Phys. Lipids, 1996, 84: 165-173; Jira, W. et al., Biosci, 1998, 53: 1061-1071). Normally, superoxide radicals generated in the cell's electron...
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