Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

MRI molecular image probe and its preparing method

A molecular imaging and probe technology, used in the analysis of materials, material analysis through resonance, sensors, etc., can solve the problems of specific instability, increase the complexity of synthesis, poor penetration and permeability, etc., to improve penetration. properties, improve biological performance, and the effect of good use effect

Inactive Publication Date: 2007-07-18
PEOPLES HOSPITAL PEKING UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with PET technology, nuclear magnetic resonance imaging molecular imaging technology has the advantages of non-radioactive probes and high anatomical resolution of images; but the disadvantage is low signal detection and specificity
This is mainly due to the following problems in antibodies and specific monoclonal antibody probes: antibodies need to be desensitized when used; antibodies, including specific monoclonal antibodies, are still unstable in vivo, and it is difficult to meet expectations The purpose; the antibody has a large molecular weight and poor penetration and permeability in the tissue, so the best imaging time is long
This obviously increases the complexity of synthesis and the cost of making the probe, making the MRI probe lose the value of actual clinical use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MRI molecular image probe and its preparing method
  • MRI molecular image probe and its preparing method
  • MRI molecular image probe and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, HYNIC and Tricine and the MRI probe of paramagnetic metal Gd

[0040] 1. GdCl 3 Synthesis: take 36.3mg Gd 2 o 3 , add 10ml of 0.1mol / L HCl, and heat to 60°C. Continue to react for 10 minutes until it is completely dissolved, then heat to 100°C to evaporate excess HCl.

[0041] 2. Preparation of chelating agent solution: 3 mg [HYNIC-D-Phe 1 ,Tyr 3 ]-Octreotide (HYNIC-TOC, synthesized by Shanghai Gil Biochemical Company) was dissolved in 8ml 0.2M Na 2 HPO 4 In PH6-7 buffer solution, after fully dissolving, take 20mg Tricine and 50mg mannitol and dissolve them in the above solution, and heat in a water bath for 10 minutes.

[0042] 3. Preparation of MRI probe: take the GdCl synthesized in the first step 3 (3-4 μmol) was added to the above-mentioned chelating agent solution for chelating reaction, shaken and mixed at room temperature, and the product was separated by HPLC to obtain the Gd-Tricine-HYNIC-Octreotide molecular probe.

[0043] The molecul...

Embodiment 2

[0052] Embodiment 2, HYNIC and EDDA and the MRI probe of paramagnetic metal Gd

[0053] 1. GdCl 3 Synthesis: take 36.3mg Gd 2 o 3 , add 10ml of 0.1mol / L HCl, and heat to 60°C. Continue to react for 10 minutes until it is completely dissolved, then heat to 100°C to evaporate excess HCl.

[0054] 2. Prepare chelating agent solution: mix 3mg [HYNIC-D-Phe 1 ,Tyr 3 ]-Octreotide (HYNIC-TOC) dissolved in 8ml 0.2M Na 2 HPO 4 In PH6-7 buffer solution, after fully dissolving, take 40mg EDDA and 50mg mannitol and dissolve them in the above solution, and heat in a water bath for 10 minutes.

[0055] 3. Preparation of MRI probe: take the GdCl synthesized in the first step 3 (3-4 μmol), added to the above-mentioned chelating agent solution for chelating reaction, shaken and mixed at room temperature, and the product was separated by HPLC to obtain Gd-EDDA-HYNIC-Octreotide molecular probe.

[0056] Through animal experiments, it has the same performance as the Gd-Tricine-HYNIC-Octr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A MRI molecular image probe with high biologic performance and low cost is composed of central ion, specific molecule and chelating agent consisting of primary chelating agent chosen from HYNIC and secondary one chosen from Tricine and EDDA. Its preparing process is also disclosed.

Description

technical field [0001] The invention relates to an MRI molecular imaging probe and a synthesis and preparation method thereof. Background technique [0002] Magnetic resonance imaging (MRI) technology is one of the three major technologies of medical molecular imaging, and others include PET (SPECT) and optical imaging. Compared with PET technology, nuclear magnetic resonance imaging molecular imaging technology has the advantages of non-radioactive probes and high anatomical resolution of images; however, the disadvantages are low signal detection and specificity. At present, MR is still in the category of anatomical morphology diagnosis. Functional MR and MR spectroscopy can only be classified into the early stage of indirect molecular imaging. MR molecular imaging has the potential to become the most promising molecular imaging method. What is lacking now is the direct molecular imaging method. Targeted specific probes. [0003] Probes are the basis of magnetic resonanc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61B5/055G01R33/20
Inventor 杜湘珂霍天龙张森李绪斌
Owner PEOPLES HOSPITAL PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com