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Method for mixed bacterial strain to degrade and ferment cellulose for producing hydrogen

A technology for degrading cellulose and cellulose, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of low utilization rate of cellulose, low hydrogen production, and slow hydrogen production rate.

Inactive Publication Date: 2007-08-01
HARBIN INST OF TECH
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Problems solved by technology

[0003] The purpose of the present invention is to solve the defects of low cellulose utilization rate, slow hydrogen production rate and low hydrogen production in the existing technology of microbial fermentation of straw lignocellulose to produce hydrogen, and to provide a strain compound degradable fiber Method for hydrogen production by vegetarian fermentation

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  • Method for mixed bacterial strain to degrade and ferment cellulose for producing hydrogen

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specific Embodiment approach 1

[0006] Specific embodiment 1: The method for producing hydrogen by fermentation of strains compounded to degrade cellulose in this embodiment is carried out according to the following steps: (1) Degrading cellulose by degrading hydrogen-producing bacteria X 9 and efficient hydrogen producing bacteria B 49 Inoculate in the fermentation broth with cellulose as the fermentation substrate; (2) carry out anaerobic fermentation in an environment with a temperature of 35-42°C, a pH value of 3.0-9.0, and high-purity nitrogen as a carrier gas, and ferment for 4-4 8h, a large amount of hydrogen can be obtained.

[0007] Cellulose-degrading hydrogen-producing strain X in this embodiment 9 It is obtained by separating and purifying from the activated sludge of "Organic Wastewater Fermentation Biological Hydrogen Production Equipment" with the patent number 92114474.1, the application date is December 12, 1992, and the authorized announcement number is CN1031392C. First, take the activat...

specific Embodiment approach 2

[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step (1), the cellulose-degrading hydrogen-producing bacteria X 9 The concentration of the pure culture solution is 571.4g / L, and the high-efficiency hydrogen-producing bacteria B 49 The concentration of the pure culture solution is 577.4g / L, and the cellulose-degrading hydrogen-producing bacteria X 9 and efficient hydrogen producing bacteria B 49 The amount of inoculum used was to inoculate 8-12 mL of pure culture liquid per liter of fermentation liquid. Other steps are the same as those in Embodiment 1.

specific Embodiment approach 3

[0011] Specific embodiment three: the difference between this embodiment and specific embodiment two is: cellulose-degrading hydrogen-producing bacteria X 9 and efficient hydrogen producing bacteria B 49 The amount of inoculum was 10 mL of pure culture liquid per liter of fermentation liquid. Other steps are the same as the second embodiment.

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Abstract

The invention relates to a method for degrading cellulose and generating hydrogen through fermentation with composited bacteria. It solves problems of low availability ratio, slow hydrogen generation speed, low hydrogen generation in current bacteria fermentating straw cellulose hydrogen generating technology. The method comprises following steps: (1) inoculating cellulose degradation and hydrogen generation bacteria X9 and high- effective hydrogen generation bacteria B49 into fermentating liquid which takes cellulose as substrate; (2) carrying out anaerobic fermentation at 35- 42 Deg. C, pH 3.0- 9.0 and with nitrogen gas as carrier gas for 4- 8 hours, and getting a large amount of hydrogen. The invention is characterized by simple operation, convenient managementm high hydrogen generation YH2 1760- 1810.3 ml per volume fermentation liquid, hydrogen generating speed of 51.2- 55.4 mmolH2 / gdrycell. H and high cellulose availability ratio of 74.5- 77.6%.

Description

technical field [0001] The invention relates to a method for degrading cellulose and fermenting hydrogen production. Background technique [0002] As a clean high-grade secondary energy resource, hydrogen has attracted more and more attention from all over the world. Among various hydrogen production methods, the use of microorganisms to ferment biomass (organic wastewater, straw agricultural and forestry waste, organic waste, etc.) to produce hydrogen has multiple advantages such as pollution control, environmental protection and production capacity, and has broad development prospects. research topics of interest. Among them, the research on the use of straw-like lignocellulose and other cheap biomass to ferment hydrogen is particularly attractive, but the existing technology of microbial fermentation low-volume defects. Contents of the invention [0003] The purpose of the present invention is to solve the defects of low cellulose utilization rate, slow hydrogen prod...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12P39/00C12N1/20C12R1/01
Inventor 王爱杰任南琪史英君林明包红旭于振国赵阳国
Owner HARBIN INST OF TECH
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