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Method of extracting reserpine from davilpepper

A technology of reserpine and Rauwolfia, applied in the field of extracting reserpine from Rauwolfia, can solve the problems of flammability, high benzene toxicity, and inability to extract wet materials, etc., and achieves the effects of less pollution and simple equipment

Inactive Publication Date: 2007-08-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process can remove a large amount of fat-soluble impurities, which is convenient for operation and does not require high equipment. However, this process uses a large amount of benzene and potassium thiocyanate. Benzene is highly toxic, flammable, and insoluble in water, which is harmful to the environment and safety. The impact is more serious, and potassium thiocyanate is also very toxic, and this process can only be used for the extraction of dry materials, but not wet materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 2500g of dry emetic Rauwolfia root powder, add 40% ethanol solution containing 0.2mol / L acetic acid to extract, filter, and extract twice, each extraction time is 5h, stirring intermittently during extraction, twice The volumes of the liquids added in the extraction are 25000ml and 12500ml respectively. The two extracts are heated and concentrated under reduced pressure, evaporated to remove ethanol, filtered, and the filtrate is used as the original adsorption solution. 125ml of Diaion HP-20 non-polar macroporous adsorption resin (Mitsubishi Chemical Co., Ltd., Japan) in the solution was used for adsorption, and then the adsorption stock solution extracted for the first time was added for adsorption, and the total adsorption was 10h, and then 380ml of deionized water was used to pass into the resin Wash the column for 0.5h, and use 125ml of 70% ethanol solution (containing 0.5mol / LHNO 3 ) into the resin column for elution, the elution time is 3h, the eluent is div...

Embodiment 2

[0025] Take 2500g of dry emetic Rauwolfia root powder, add 0.02mol / L H 2 SO 4 90% ethanol solution leaching, filtering, total leaching 3 times, each leaching time is 2h, stirring intermittently during leaching, the liquid volumes added in 3 times leaching are respectively 25000ml, 12500ml, 7500ml, and 3 parts The extraction solution is heated and concentrated under reduced pressure respectively, evaporates ethanol, filters, and the filtrate is used as the adsorption stock solution, and the adsorption stock solution of the third leaching is first passed into the 500ml Amberlite XAD-4 nonpolar macroporous adsorption resin ( U.S. Rohm-Haas Company) is adsorbed, then the adsorption stock solution of the second leaching is introduced for adsorption, and finally the adsorption stock solution of the first leaching is introduced for adsorption, and the adsorption is total for 20h, and then passed through with 1500ml deionized water Wash in the resin column for 0.5h, and use 2000ml 90...

Embodiment 3

[0027] Take 2500g of dried Indian Rauwolfia root powder, add 0.05mol / L H 2 SO 4Extract with 50% ethanol solution, filter, and co-extract twice, each extraction time is 3h, stirring intermittently during extraction, the liquid volumes added in the two extractions are 25000ml and 12500ml respectively, and the two extractions The solution was heated and concentrated under reduced pressure, evaporated to remove ethanol, filtered, and the filtrate was used as the adsorption stock solution. The adsorption stock solution extracted for the second time was first passed into the 200ml HP-20 macroporous adsorption resin installed in the column for adsorption, and then passed into the second adsorption stock solution. The adsorption stoste of once leaching, absorbs altogether 10h, passes in the resin column with 1000ml deionized water to wash 1h then, with 1000ml 80% ethanol solution (containing 0.05mol / L H 2 SO 4 ) into the resin column for elution, the elution time is 6h, the eluent i...

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PUM

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Abstract

The invention discloses an extracting method of reserpine from davilpepper in the medical technical domain, which comprises the following steps: adding powder of davilpepper root into alcohol; stirring; leaching; filtering to obtain leached liquid; repeating those procedures; decompressing each leached liquid to heat and condense; adopting non-polarity large-hole adsorbing resin to adsorb; washing through water; eluting through alcohol; collecting elution segment by segment; combining reserpine with content over 0.4g / L as total elution; decompressing; heating; condensing; adding water and alkaline; stirring; obtaining solid alkaloid; combining all solid alkaloid to dissolve in the acetone; adding composite solution of chloroform-ligarine; stirring; sedimenting colloid; decompressing supernatant; heating; condensing; obtaining the solid; adding the solid into organic solvent to crystallize; obtaining the product.

Description

technical field [0001] The invention relates to a medicine separation method in the technical field of medicine, in particular to a method for extracting reserpine from Rauwolfia. Background technique [0002] The plant Rauwolfia contains various alkaloids such as reserpine, amarin, and yohimbine. Reserpine mainly exists in the root of Rauvolwolfia and has the function of lowering blood pressure. It is an important raw material for the production of antihypertensive drugs in the pharmaceutical industry. [0003] Found through retrieval to prior art literature, " the research of the active ingredient of Chinese herbal medicine (first volume), extraction, separation, identification and determination of content " (Beijing: People's Medical Publishing House, 1972 first volume) edited by Institute of Materia Medica, Chinese Academy of Medical Sciences edition, pages 200-201), a process for extracting reserpine from Rauwolfia is proposed, which is as follows: take the root powder...

Claims

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Application Information

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IPC IPC(8): C07D459/00A61K36/24A61K125/00
Inventor 赵凤生冯蕾孙蕾
Owner SHANGHAI JIAO TONG UNIV
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