Method of separating and purifying ursolic acid and oleanolic acid

A technology for separation and purification of oleanolic acid, which is applied in the fields of organic chemistry, steroids, sugar derivatives, etc., can solve the problems of low separation efficiency, complicated separation and purification technology, etc., achieves good chemical stability, and simplifies extraction steps. , The effect of chemical stability and antimicrobial ability

Inactive Publication Date: 2007-08-15
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is: in order to overcome the deficiencies such as existing separation and purification technology is complicated and separation efficiency is no...

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  • Method of separating and purifying ursolic acid and oleanolic acid

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Experimental program
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Effect test

experiment example 1

[0027] A) Get 5 grams of Kudingcha powder, add 100mL of pure water as the extract, extract in a boiling water bath for 1 hour, filter, and use 100mL of absolute ethanol solution as a solvent to extract the two residues obtained by filtering in a boiling water bath. Once, each time for 2 hours, the extract was filtered, and the filtrate was clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 3m / s and an operating pressure of 0.18MPa. The concentration of ursolic acid in the clarified liquid was detected to be 0.428mg / mL, the concentration of oleanolic acid is 0.149mg / mL, and the clarified solution is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 400 to obtain a concentration of ursolic acid of 3.878mg / mL in the concentrated solution, and a concentration of oleanolic acid of 1.246mg / mL;

[0028] B) The concentrated solution is injected into the macroporous adsorption resin chromato...

experiment example 2

[0031] A) Take 3 grams of Kudingcha powder, add 100mL of pure water as the extract, extract in a boiling water bath for 1 hour, filter, and use 100mL of absolute ethanol solution as a solvent to extract the filter residue obtained by reflux in a boiling water bath for three times , each time for 2 hours, the extract was filtered, and the filtrate was clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 2.5m / s and an operating pressure of 0.15MPa. The concentration of ursolic acid in the clarified liquid was detected to be 0.286mg / mL, the concentration of oleanolic acid is 0.101mg / mL, and the clarified solution is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 300 to obtain a concentration of ursolic acid of 2.437mg / mL in the concentrated solution, and a concentration of oleanolic acid of 0.923mg / mL;

[0032] B) The concentrated solution is injected into the macroporous adsorption r...

experiment example 3

[0035] A) Take 5 grams of Kudingcha powder, add 100mL of pure water as the extract, extract in a boiling water bath for 1 hour, filter, and use 100mL of absolute ethanol solution as a solvent to extract the filter residue obtained by reflux once in a boiling water bath , 2 hours each time, the extract was filtered, and the filtrate was clarified with a 500nm ceramic membrane at an operating temperature of 40°C, a membrane surface flow rate of 2m / s and an operating pressure of 0.1MPa. The concentration of ursolic acid in the clarified liquid was detected to be 0.147mg / mL, the concentration of oleanolic acid is 0.191mg / mL, and the clarified solution is concentrated 10 times by a nanofiltration membrane with a molecular weight cut-off of 500 to obtain a concentration of ursolic acid in the concentrated solution of 1.025mg / mL and a concentration of oleanolic acid of 1.306mg / mL. mL;

[0036] B) The concentrated solution is injected into the macroporous adsorption resin chromatogra...

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Abstract

The invention discloses an extracting method of urson and oleanolic acid from bitter leaves, which comprises the following steps: adopting bitter leaves from Hainan as raw material; extracting through pure water; filtering; refluxing filter slag to extract through alcohol; adopting inorganic film filtering method to remove macromolecular impurity in the extract; hyperfiltering; condensing the penetration liquid through nanofiltering; adsorbing and eluting through macromolecular resin chromatographic column; condensing eluent to crystallize; obtaining rough product of total triterpenic acid; washing the rough product; dissolving in the alcohol; alkalining; acidifying; washing again; recrystallizing; obtaining the total triterpenic acid; dissolving the total triterpenic acid in the carbinol to boil; filtering; condensing filtrate; evolving; obtaining the monomer product of oleanolic acid; washing the sediment; dissolving the crystal through alcohol; obtaining the monomer product of urson; hyperfiltering and clarifying filtrate of bitter leaves; nanofiltering; condensing; adsorbing through polyamide resin selectively; eluting; condensing the eluent; obtaining the monomer of tea polyphenol with certain purity.

Description

technical field [0001] The invention belongs to the technical field of drug separation. Specifically, it relates to a method for extracting and purifying the main active components ursolic acid and oleanolic acid from Kudingcha, and simultaneously obtaining tea polyphenol by-products with a certain purity. Background technique [0002] Kudingcha is a traditional herbal tea in our country. It has been drunk in my country for more than 2000 years. , enhancing immune function, anti-stress, anti-fatigue, antibacterial, health care and medicinal functions of trace elements, antagonizing the contraction of bronchial smooth muscle, toxicological effects, etc. Good development prospects. [0003] The composition of Kudingcha is relatively complex. In addition to the same ingredients as ordinary tea such as tea polyphenols, amino acids, catechins, glycolipids, and vitamins, it also contains more triterpenoid saponins, such as the main active ingredient Ursolic acid and oleanolic aci...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07D311/58C07D311/22C07H17/06
Inventor 邢卫红徐南平王珏
Owner JIANGSU JIUWU HITECH
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