Method for inspecting blood conservative-liquid intracellular toxin of blood-collecting bag

A technology for blood preservation solution and bacterial endotoxin, which is applied to measurement devices, preparation of test samples, instruments, etc., can solve the problems of increased probability of blood preservation solution being polluted by the environment, high sensitivity of limulus reagents, and unstable test results. , to achieve the effect of simple facilities and equipment, less chance of environmental pollution, and stable test results

Inactive Publication Date: 2007-09-05
邯郸市中心血站
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Problems solved by technology

The BET detection method is suitable for blood centers or blood stations, but because the citrate ions in the blood preservation solution will interfere with the Limulus test, it is often diluted with water to eliminate interference, but it has many steps and large errors, which will cause Increased chance of environmental contamination of blood preservation solution and unstable test results. At the same time, due to the increase in water addition, high sensitivity of LAL reagent is required, which increases the cost.

Method used

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Embodiment Construction

[0010] Embodiments of the present invention utilize calcium hydroxide [Ca(OH) 2 ] High temperature resistant physical and chemical properties, take a small amount of analytically pure Ca(OH) 2 Put it in a clean glass container, dry-bake it in an oven at 250°C for more than 1 hour to remove pyrogens, then add pyrogen-free water to dissolve to make a saturated solution, take the supernatant of the solution and dilute the blood preservation solution 11 times, and then use Dilute 2 times with pyrogen-free water to obtain a 22-fold dilution of the blood preservation solution, and then use the limulus reagent with a sensitivity of 0.25 EU / ml, according to method 1 (gel method) in the 2005 edition of "Chinese Pharmacopoeia" Appendix XI E bacterial endotoxin inspection method ) for detection.

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Abstract

A method for detecting bacterial toxins of blood storage liquid in blood sampling bag includes taking Ca(OH)2 and drying it under high temperature to remove off heat source, preparing it to be saturated solution, adding blood storage liquid to dilute said saturated solution to be diluted solution of certain times and carrying out detection by utilizing king crab reagent according to gel method listed in bacterial toxins detection manner of XIE appendit on 2005 version of Chinese pharmacopeia.

Description

technical field [0001] The invention relates to a method for detecting bacterial endotoxin in a blood preservation solution of a blood collection bag. Background technique [0002] Bacterial endotoxins in the blood preservation solution in the blood collection bag can cause pyrogen reactions in patients. The current national regulations and standards "Chinese Pharmacopoeia" and "National Drug Standards" stipulate that the limit value of bacterial endotoxin in the blood preservation solution is <5.56EU / ml, and stipulates two detection methods for detecting pyrogens or bacterial endotoxins, namely the rabbit method and the Limulus Reagent Bacterial Endotoxin Test (BET) method. If the rabbit method is used for detection, it must be equipped with animal rooms and animals that meet the experimental standards, as well as the feeding and management personnel of experimental animals and a series of supporting facilities. The cost is relatively high, which is suitable for blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/579G01N1/38
Inventor 王洪段现民赵有良牛海江
Owner 邯郸市中心血站
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