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Gram-negative bacteria microcapsule and preparation method thereof

A technology of gram-negative bacteria and microcapsules, which is applied to bacteria, fixed on/in organic carriers, etc., can solve the problems of difficult storage and transportation, inconvenience, and decreased activity of liquid inoculants, and achieve good storage stability. Sex, the effect of strong growth and reproduction ability

Inactive Publication Date: 2007-10-31
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Gram-negative bacteria is an efficient petroleum-degrading bacterium screened and domesticated from petroleum-contaminated soil. The impact of other factors is serious, the activity declines rapidly, and the liquid bacterial agent is difficult to store and transport, and it is also extremely inconvenient in practical application. However, the commonly used methods of bacteria preservation, such as semi-solid puncture preservation method, inclined plane preservation method, sand tube Preservation method, low-temperature cryopreservation method, etc., have strict requirements and high cost, and are not suitable for large-scale use

Method used

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  • Gram-negative bacteria microcapsule and preparation method thereof

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Effect test

Embodiment 1

[0009] Prepare a colloidal solution at 30°C according to the mass ratio of wall materials: gum arabic: maltodextrin: β-cyclodextrin: carboxymethyl cellulose 1:9:0.5:0.2, add 150ml of culture medium and stir evenly. Use a peristaltic pump to put the material into the atomizer for spray drying. The spray drying inlet air temperature is 140°C, the material feed flow rate is 6ml / min for spray drying, and the outlet air temperature is 50°C. The particle size range is 10μm-100μm. The microcapsule product has a live bacteria rate of 75.33%, and after eight months of storage, the order of live bacteria still reaches 10 9 pcs / g.

Embodiment 2

[0011] Prepare a gel solution at 30°C according to the mass ratio of wall materials: gum arabic: maltodextrin: β-cyclodextrin: carboxymethyl cellulose 1:9:0.5:0.2, add 150ml of culture medium and stir evenly . Use a peristaltic pump to put the material into the atomizer for spray drying. The spray drying air temperature is 160°C, and the material feed flow rate is 6ml / min for spray drying to obtain microcapsule products with a particle size range of 10 μm-100 μm. The rate of viable bacteria can reach 68.99%, and after eight months of storage, the order of viable bacteria still reaches 10 9 pcs / g.

Embodiment 3

[0013] Prepare a gel solution at 30°C according to the mass ratio of the wall material: gum arabic: maltodextrin:: β-cyclodextrin: carboxymethyl cellulose: 1:9:0.5:0.2, add 150ml of culture medium, Stir well. Use a peristaltic pump to put the material into the atomizer for spray drying. The spray drying inlet air temperature is 140°C, and the material feed flow rate is 15ml / min for spray drying to obtain microcapsule products with a particle size range of 10 μm-100 μm. The rate of viable bacteria can reach 71.69%, and after eight months of storage, the order of viable bacteria still reaches 10 9 pcs / g.

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Abstract

The invention discloses a preparing method of Gram-negative bacterium microcapsule in microcapsule production technical domain, which is characterized by the following: choosing acacia gum, malt dextrin, beta-cyclodextrin and carboxymethyl cellulose as wall material; sieving and domesticating Gram-negative bacterial liquid from earth oil polluted soil; using as core material; naming bacterial agent as barley sugar oligotrophy single cell bacteria; adopting spray drying method; preparing microcapsule with grain size at 10mum-100mum; making live bacteria ratio reach 84. 57%; storing for eight months under the condition of 18-30 deg. c; reaching the live bacteria magnitude order at 109 separate / g; lengthening retention period largely. This invention possesses the advantages of low cost, simple craft, convenient operation and strong practicability, which is easy to realize large scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of preparation of microcapsules, and relates to a Gram-negative bacteria microcapsule and its preparation method. The technique of preparing microcapsules by using the spray drying method coats the oil-contaminated soil bioremediation bacterial agent-Gram-negative bacteria in the particle size A new microcapsule product is formed in the tiny wall material. Background technique [0002] Gram-negative bacteria is an efficient petroleum-degrading bacterium screened and domesticated from petroleum-contaminated soil. The impact of other factors is serious, the activity declines rapidly, and the liquid bacterial agent is difficult to store and transport, and it is also extremely inconvenient in practical application. However, the commonly used methods of bacteria preservation, such as semi-solid puncture preservation method, inclined plane preservation method, sand tube Preservation method, low temperature cryopr...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N1/20
Inventor 于才渊贾凌云王宝和韩冬冬
Owner DALIAN UNIV OF TECH
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