Bacteria nano magnetosome loaded with primary amino medicine and preparation method thereof

A technology with primary amine group and nano-magnetism, which can be used in drug combinations, pharmaceutical formulations, medical preparations with non-active ingredients, etc. effect, drug and carrier separation, etc., to achieve the effect of easy separation and purification, not easy to aggregate, and high drug loading

Inactive Publication Date: 2007-11-28
CHINA AGRI UNIV
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the stability of most drug-loaded nanoparticles is not good, especially in the body, it is easily degraded by various environmental factors such as water, pH, temperature, ionic strength and various biomolecules, so that the drug is separated from the carrier, Lost the original controllable release and targeting effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacteria nano magnetosome loaded with primary amino medicine and preparation method thereof
  • Bacteria nano magnetosome loaded with primary amino medicine and preparation method thereof
  • Bacteria nano magnetosome loaded with primary amino medicine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of bacterial nano-magnetosomes loaded with doxorubicin

[0038] (1) Suspend 1 mg of pure bacterial nano-magnetosomes in 10 ml of distilled water, using 60 Co irradiation sterilization (irradiation dose is 1-3kGy), then magnetosomes are adsorbed with a magnet, and the supernatant is removed;

[0039] (2) Wash the adsorbed magnetosomes three times with sterile PBS solution, then suspend them with 1ml sterile PBS solution, add 100ml filter-sterilized 1mg / ml doxorubicin (purchased from Shenzhen Junning Technology Co., Ltd. ) solution, ultrasonically disperse for 1 minute, then add 0.2 μl of 50% glutaraldehyde (purchased from Sigma Company) solution, mix well, and ultrasonically 2 times to the mixed solution (20 minutes for each ultrasonic wave, 1 minute in between, in this embodiment The ultrasonic treatment parameters are the same) and then let it stand still. After the suspension is stratified, the magnetosomes are adsorbed, and the supernatant is r...

Embodiment 2

[0041] Example 2 Preparation of bacterial nano-magnetosomes loaded with doxorubicin

[0042] (1) Suspend 100 mg of pure bacterial magnetosomes in 100 ml of distilled water, using 60 Co irradiation sterilization (irradiation dose is 10-15kGy), then magnetosomes are adsorbed with a magnet, and the supernatant is removed;

[0043] (2) Wash the adsorbed magnetosomes 5 times with sterile PBS solution, then suspend them with 100ml sterile PBS solution, add 1ml filter-sterilized 1mg / ml doxorubicin solution, ultrasonically disperse for 20 minutes, then add 80μl 50% glutaraldehyde solution, mixed well, the mixed solution was ultrasonicated 100 times (1 minute for each ultrasonic wave, 60 minutes interval between every two times, the ultrasonic treatment parameters were the same in the present embodiment) and left standing, until the suspension was separated Adsorb the magnetosomes after layering, remove the supernatant, resuspend the precipitate with sterile PBS solution, absorb the m...

Embodiment 3

[0045] Example 3 Preparation of bacterial nano-magnetosomes loaded with doxorubicin

[0046] (1) Suspend 20 mg of pure magnetosomes in 100 ml of distilled water, using 60 Co irradiation sterilization (irradiation dose is 10-15kGy), then magnetosomes are adsorbed with a magnet, and the supernatant is removed;

[0047] (2) Wash the adsorbed magnetosomes three times with sterile PBS solution, then suspend them with 70ml sterile PBS solution, add 30ml filter-sterilized 1mg / ml doxorubicin solution, ultrasonically disperse for 5 minutes, and then add 1ml 50% glutaraldehyde solution, mixed well, the mixed solution was sonicated 14 times (1 minute for each sonication, 4 minutes between every two times, the sonication parameters were the same in the present embodiment) and left standing, until the suspension was separated Adsorb the magnetosomes after layering, remove the supernatant, resuspend the precipitate with sterile PBS solution, absorb the magnetosomes after ultrasonic dispers...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides one kind of nanometer bacterial magnetic corpuscle carrying primary amino group medicine and its preparation process. The nanometer bacterial magnetic corpuscle consists of three structural units, including homotype double-function reagent, primary amino group medicine and nanometer bacterial magnetic corpuscle. The nanometer bacterial magnetic corpuscle carrying primary amino group medicine has high medicine carrying capacity, less aggregation, easy separation and purification, anticancer and antitumor effect similar to that of pure primary amino group medicine, but much lower toxicity.

Description

technical field [0001] The invention relates to the field of bacterial nano-magnetosomes, in particular to a bacterial nano-magnetosome carrying primary amine-based drugs and a preparation method thereof. Background technique [0002] Nano-drug carriers refer to nanoparticles used to load (adsorb, link or embed) drugs, and their diameters are generally less than 100nm. The particle size of the nanometer drug carrier is small, which can increase the permeability of the drug to the biomembrane, and is conducive to the transdermal absorption of the drug and the exertion of the drug effect in the cell. The nano-medicine carrier has a large specific surface area, which can increase the contact area between the drug and the body and improve the utilization of the drug. [0003] The magnetic nano-drug carrier can be guided to the target site under the action of an external magnetic field to realize targeted drug delivery. Targeted drug administration can greatly simplify the way ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/46A61K9/14A61K31/704A61P35/00A61K47/54
Inventor 李颖孙建波姜伟田杰生王珍芳李季伦
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap