Method for taking off endotoxin in primary pure plasmids or proteins, and kit

A protein and endotoxin technology, applied in the field of biomedicine, can solve the problems of unreachable, affected yield, and poor removal of plasmid endotoxin.
CN101091797AInactive Publication Date: 2007-12-26SHANGHAI H & G BIOTECH +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI H & G BIOTECH
Publication Date
2007-12-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a method for removing endotoxin from primary pure plasmid or protein. Said method includes the following steps: independently using PMXB or joint-using PMXB and TritonX-114, adding organic solvent, mixing them, making phase separation, or firstly using PMXB and Tritonx-114 and adding organic solvent to make phase separation, the adopting combination method of PMXB gel mixing method and / or PMXB gel chromatography to remove endotoxin from plasmid or protein.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to the purification process of plasmids used in gene vaccines and gene therapy and the purification process of proteins, more specifically, to a method for removing endotoxins in primary purified plasmids or proteins. Background technique

[0002] Nucleic acid vaccines using "naked engineering plasmids" are the latest or third-generation vaccines that have emerged in the past ten years and are currently being developed. Studies have shown that they are basically ineffective against infectious diseases such as caries, AIDS, The prevention of tuberculosis and malaria and the treatment of hepatitis B have unique advantages and application prospects. The unique advantages of nucleic acid vaccines in inducing the body's cellular immune response make up for the shortcomings of the first and second generation vaccines, and these remarkable advantages are necessary for the prevention and treatmen...

Claims

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