Experimental apparatus for loading cell through digital controlled mechanical strain

Abstract

This invention discloses a cell loading experimental machine by numerically controlled mechanical strain. The cell loading experimental machine comprises a computer, a controller, and a mechanical strain device. The controller adopts a single chip microcomputer to control the stepping motor with photoelectric encoder displacement feedback. The stepping motor drives the precision cam transmission system, which then drives the semispherical top plate to move to-and-from. The semispherical top plate top-presses the elastic cell culture dish so that cells are subjected to mechanical stretching strain. The cell loading experimental machine can apply dynamic mechanical stretching strains with different strengths, frequencies and times to the cells cultured in vitro, and has such advantages as wide strain output range, high precision, easy operation and direct display.

Description

technical field [0001] The invention relates to a numerically controlled mechanical strain cell loading experiment machine, which belongs to the field of cell mechanics and is used for applying mechanical strain to cells cultured in vitro to study the effect of mechanical strain on cells. Background technique [0002] Cell mechanics is the frontier of the development of modern biomechanics. It mainly studies the influence of mechanical load on cell morphology, growth and differentiation, and function. It is one of the foundations of tissue engineering and cell engineering. Due to the extremely complex environment in vivo, it is difficult to distinguish the effects of mechanical factors and other factors on cells in vivo cell mechanics experiments. Therefore, mechanical loading experiments on cells cultured in vitro are an important method for cell mechanics research. [0003] The basis and key of cell mechanics research is the research of cell mechanics loading technology an...

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Problems solved by technology

[0005] Foreign scholars Hasegawa S, Sato S, Saito M, Suzuki Y, Brunette DM published an article entitled Mechanical stretching increases the number of cultured bone cells synthesizing DNA and alters their pattern of protein synthesis in "Calcified tissue international" volume 37 pages 431-436 , an in vitro cell mechanical loading device based on the circular substrate deformation method is described in this paper. Weights are used to apply continuous or intermittent stretching to the elastic membrane with cells attached to the convex platen. The degree of membrane stretching varies with the pressure plate used. The curvature varies with different types of cells, but the device cannot dynamically load the cells, and the device cannot be automatically controlled by a computer, and it is difficult to precisely adjust the amount of strain loaded on the cells, which limits the application of the device in the study of cell mechanics in vitro

Foreign scholars Banes AJ, Gilbert J, Taylor D, Monbureau 0 published a paper titled A new vacuum-operatedstress-providing instrument that applies static or variable durat ion cyclic tension or compress ion to cells in "Journal of cellscience" volume 75, pages 35-42 Invitro's article also describes an in vitro cell mechanical loading device based on the circular base deformation method, which uses the negative pressure generated by vacuum to deform the bottom of the cell culture dish to mechanically load the cells. Perform dynamic loading, but the device cannot be automatically controlled by computer, and the amount of strain loaded on the cells is very small, and the maximum strain can only be loaded to 0.13% of the cells, thus limiting its application range in in vitro cell mechanics research

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