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Biochemical inspection device and biochemical inspection method

An inspection device and inspection method technology are applied in the field of devices for analyzing biochemical reactions, and can solve problems such as reducing and increasing the burden on inspectors, failing to obtain fluorescence image inspection efficiency, and the like

Inactive Publication Date: 2008-01-23
OLYMPUS CORP
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, in this method, for example, when the luminescence intensity of each array element of the array for biochemical testing is significantly reduced due to the wrong solution of the biochemical substance by the examiner, a large amount of accumulation time (for example, 300 seconds), which not only increases the burden on the examiner, but also leads to a decrease in examination efficiency in which the required fluorescence image cannot be obtained even after waiting for 300 seconds

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  • Biochemical inspection device and biochemical inspection method
  • Biochemical inspection device and biochemical inspection method
  • Biochemical inspection device and biochemical inspection method

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Embodiment Construction

[0024] Hereinafter, embodiments of the present invention will be described with reference to the drawings.

[0025] FIG. 1 shows the configuration of a biochemical testing device according to an embodiment of the present invention. In FIG. 1 , the excitation light source 204 is various lamps such as a mercury light source or an LED, and is connected to a power supply unit 206 . A shutter unit 208 incorporating a shutter 208 , a lens 210 , a filter unit 212 incorporating two excitation filters 212 a , and a dichroic mirror 214 are disposed on the optical path of excitation light emitted from the excitation light source 204 . An objective lens 216 and a stage 202 on which the array 100 for biochemical inspection is placed are arranged on the reflection optical path of the dichroic mirror 214 . In addition, an imaging lens 218 and a CCD camera 220 serving as an imaging element are disposed on the transmission optical path of the dichroic mirror 214 . The CCD camera 220 includes...

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Abstract

Firstly, imaging is performed with an exposure time of ExpStart. When the maximum intensity value of all the pixels in the image is smaller than TransStart, the exposure time is increased and imaging is performed again. After this, increase of the exposure time and imaging are repeatedly performed until the maximum intensity value of all the pixels of the imaged optical image reache the TransStart. When the maximum intensity value has reached TransStart, the image is transferred to a computer and recorded on a hard disc. The number of recorded discs is counted. After this, increase of the exposure time, imaging, image data transfer and recording, and the number of recorded discs are repeatedly performed. The imaging is terminated when the minimum intensity value in an area smaller than the entire area of the optical image has become greater than TransEnd or the number of recorded discs has become greater than TransPicts or the exposure time has become greater than MaxExpTime.

Description

technical field [0001] The present invention relates to devices and methods for analyzing biochemical reactions. Background technique [0002] In recent years, genetic analysis of various organisms including humans or various plants represented by rice has progressed. Recently, inspection methods using DNA chips or DNA microarrays in which DNA is regularly arranged in semiconductors and the like are being developed. In this examination method, multiple genes can be examined simultaneously. Regarding the same inspection method, for example, Japanese Unexamined Patent Application Publication No. 2002-350446 introduces a three-dimensional array and a prior art example of detection thereof. This document discloses a biochemical inspection method capable of simultaneously detecting light intensities corresponding to each probe array element in a practically wide dynamic range using an area sensor or a line sensor having a normal dynamic range. [0003] Japanese Patent Laid-Ope...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/17G01N21/27
CPCG01N21/6452G01N21/6456
Inventor 松尾祐一郎芝崎尊己才田有子
Owner OLYMPUS CORP
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