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Joint production method for distilling immunopotentiator from waste blood

A technology of blood cells and plasma, applied in the direction of antibodies, allergic diseases, fermentation, etc., to achieve the effect of convenient industrial production, improved utilization, safe and practical effect

Inactive Publication Date: 2008-01-30
上海汉德食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many patent documents have reported the extraction methods of SOD, Ig and STF, but they are all

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Add 10 grams of trisodium citrate to 1000 grams of fresh sheep blood, centrifuge at 3500 r.p.m. for 30 minutes to obtain 650 grams of plasma and 350 grams of blood cells;

[0034] (2) Extraction of SOD:

[0035] Wash the blood cells with a NaCl aqueous solution with a weight concentration of 0.9%, and then centrifuge at a speed of 3500r.p.m. to obtain red blood cells. Add an equal amount of water for hemolysis for 30 minutes to obtain hemolysis, and then add 3.0% weight concentration of chloride Copper aqueous solution 15ml, remove hemoglobin, centrifuge to obtain crude enzyme liquid, then concentrate to 1 / 10 of the original volume with an ultrafiltration membrane with a molecular weight cut-off of 10000, obtain ultrafiltration concentrate 40ml, add 15mg lactose to the ultrafiltrate, freeze-dry to obtain Light green freeze-dried powder 125mg SOD;

[0036] (3) SOD is measured by pyrogallol oxidation method, Pharmaceutical Industry, 1988, 19(5): 217;

[0037] (3) Ex...

Embodiment 2

[0043] (1) 10 grams of trisodium citrate was added to 1000 grams of fresh blood of cattle, centrifuged at 3000 r.p.m. for 20 minutes to obtain 600 grams of plasma and 400 grams of blood cells;

[0044] (2) Extraction of SOD:

[0045] Wash the blood cells with an aqueous NaCl solution with a weight concentration of 0.9%, and then centrifuge them at a speed of 3000r.p.m. to obtain red blood cells. Add an equal amount of water for hemolysis for 0.5 hours to obtain hemolysis, and then add chlorination with a weight concentration of 5%. 25ml of zinc aqueous solution, remove hemoglobin, centrifuge to obtain crude enzyme liquid, then concentrate it to 2 / 10 of the original volume with an ultrafiltration membrane with a molecular weight cut-off of 10,000, obtain ultrafiltration membrane concentration, add water repeatedly for ultrafiltration, and finally obtain 70ml concentrated solution, add 50mg of sucrose, freeze-dried to obtain light green freeze-dried powder 135mgSOD;

[0046] (3...

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PUM

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Abstract

The present invention discloses a co-production method of extracting the immunostimulants from waste blood, the method of the prevention can simultaneously get three products by feeding material at one time: Ig, STF and SOD, which can greatly improve the utilization rate of the blood, the ultrafiltration technology is used repeatedly during the preparation process, furthermore, the invention uses a new concept of molecular weight cutoff to separate the products one by one, the method mainly adopts the physical method and tries to avoid and add less organic solvents as much as possible, so the invention is safe, practical and good in effects. The divalent metal chloride and thermal denaturation are used in the SOD extraction process to remove the hemoglobin, so as to replace the chloroform and ethanol of the original process, further to facilitate the industrial production.

Description

technical field [0001] The invention relates to a method for extracting immune enhancer from waste blood, in particular to a method for simultaneously extracting SOD, Ig and STF from waste blood. Background technique [0002] SOD, Ig and STF are immunomodulators that can improve immune function, and all three products can be isolated from blood. [0003] At present, many patent documents have reported the extraction methods of SOD, Ig and STF, but all of them are per yield. [0004] Therefore, how to simultaneously extract SOD, Ig and STF from animal blood to improve the utilization rate and extraction efficiency of animal blood is a subject of great concern. Contents of the invention [0005] The technical problem to be solved in the present invention is to disclose a co-production method for extracting an immune enhancer from waste blood, so as to overcome the defects in the prior art. [0006] Method of the present invention comprises the steps: [0007] (1) Add tris...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K39/395A61P37/04C12P21/02
Inventor 赵健李爱国袁勤生
Owner 上海汉德食品有限公司
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