Single bilirubin quality controlled serum preparation method

A technology of bilirubin and serum, applied in biological testing, material inspection products, etc., can solve the problems of expensive imported goods, achieve the effect of prolonging the stability time and promoting antioxidant capacity

Active Publication Date: 2008-02-13
杭州美康盛德医学检验实验室有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And imports are expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Collect 150ml of serum from healthy people excluding infectious diseases and common diseases, and use it as raw serum. The above 150ml serum was divided into two 100ml plastic bottles, and placed in room temperature and -20°C refrigerator at intervals of 12 hours. After a total of 7 days, 110ml of the upper white serum was carefully separated and discarded. 110ml of serum was filtered once with ordinary dense filter paper, then transferred to 0.45μm and 0.22μm filter membranes for a total of two times to obtain 100ml of clear, transparent and sterile serum.

[0033] Take a 10ml small beaker, weigh 5mg of Bilirubin indirect bilirubin purchased from Sigma, add 1.5ml of 0.1N NaOH, mix well, and when there is no dry powder floating on the liquid surface, add 1.0ml of methanol, mix well to the solution All transparent. At this time, the total amount of liquid is 2.5ml, and 0.5ml is taken and transferred to the aforementioned 100ml of serum.

[0034] Take a small beaker wit...

Embodiment 2

[0051] Collect 150ml of serum from healthy people excluding infectious diseases and common diseases, and use it as raw serum. The above 150ml serum was divided into two 100ml plastic bottles, and placed in room temperature and -20°C refrigerator at intervals of 12 hours. After a total of 7 days, 110ml of the upper white serum was carefully separated and discarded. 110ml of serum was filtered once with ordinary dense filter paper, then transferred to 0.45μm and 0.22μm filter membranes for a total of two times to obtain 100ml of clear, transparent and sterile serum.

[0052] Take a 10ml small beaker, weigh 5mg of Bilirubin indirect bilirubin purchased from Sigma, add 1.5ml of 0.1N NaOH, mix well, and when there is no dry powder floating on the liquid surface, add 1.0ml of methanol, mix well to the solution All transparent. At this time, the total amount of liquid is 2.5ml, and 2ml is transferred to the aforementioned 100ml of serum.

[0053] Take a small beaker with a capacity...

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Abstract

The invention discloses a preparation method for quality control of serum by single bilirubin. The steps are: repeatedly freezing and thawing the serum of healthy body for 5 to 10 times in order to get the delipidized protein serum; filtering; adding 1 to 4 parts by weight of indirect bilirubin and 3 to 12 parts by weight of direct bilirubin into 100 parts by volume of filtered serum; adding 30 to 50 parts by weight of Kisumu sugar mercapto alcohol, 200 to 400 parts by weight of EDTA disodium, 3,000 to 5,000 parts by weight of lactose and 0.05 to 0.1 parts by volume of methyl-isothiazoline; adding bovine serum albumin until the albumin in system is up to 4,000 parts by weight; adjusting system pH within the range of 0.7 to 0.8 and regulating the redox electrode potential to 130mV to 150mV; packing, freezing dry and make fixed-value determination. The preparation method of the invention effectively solves the problem that bilirubin is vulnerable to heat, light and oxidation, thus guaranteeing the stability of single bilirubin determination.

Description

technical field [0001] The invention relates to a preparation method of single bilirubin quality control serum. Background technique [0002] The determination of bilirubin is one of the most commonly used items in laboratory medicine, and it is an important basis for the diagnosis and differential diagnosis of various clinical jaundice. There are many methods for the determination of bilirubin, commonly used enzymatic method, diazo reagent method and chemical oxidation method. It is generally believed that the enzymatic method is the best, because it has the advantages of strong specificity, less interference and mild reaction conditions, but the price of the enzyme is high, and there are still some problems to be solved in the methodology, so it has not been widely used yet. The diazonium reagent method has a long history, but has many disadvantages, mainly because the reagent is unstable and the reaction is unstable. The third method is chemical oxidation, which has dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/96G01N33/72
Inventor 宋克征邹炳德
Owner 杭州美康盛德医学检验实验室有限公司
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