Method for producing L-histidine

A production method, histidine technology, applied in the direction of organic chemistry, etc., to achieve low production cost, low technical difficulty, and good product quality

Active Publication Date: 2008-02-20
湖北新生源生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this invention is to provide a kind of production method of L-histidine, by researching and developing a kind of production technology that obtains L-histidine product through multiple resin selective separation, solves the mixing obtained from proteolysis Industrialization Problems of Extracting L-Histidine from Amino Acid Mother Liquor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take pig hair hydrolyzate 10m 3 , catch acid under reduced pressure, isolate L-cystine and L-tyrosine by isoelectric point precipitation, and obtain 8m mother liquor after separation 3 , put the liquid on a strongly acidic cationic resin column, use Pauly reagent to detect the effluent from the resin column, when it is orange-red, it means that there is L-histidine flowing out in the effluent, the resin is saturated, stop feeding, and elute with 0.5% ammonia water , when it is detected that L-histidine flows out, start to collect the eluate that flows out, and stop when the pH of the effluent liquid=11, and obtain a feed solution of 7.6m 3 , put the material liquid on the strong acid cationic resin column again, when it is detected that the resin is saturated, stop feeding, and elute the resin column with 1% ammonia water, after passing the detection, collect 2m of L-histidine solution with higher content 3 , neutralized with hydrochloric acid to pH = 3.6, added activa...

Embodiment 2

[0028] Take human hair hydrolyzate 10m 3 , catch acid under reduced pressure, isolate L-cystine and L-tyrosine by isoelectric point precipitation, and collect 8m mother liquor after separation 3 , put the liquid on a strong acid cationic resin column, detect the effluent from the resin column with Pauly reagent, stop feeding when it is orange-red, and elute with 0.1% ammonia water, and start to collect the effluent when L-histidine is detected to flow out The eluent stops when the effluent pH=11, and obtains the feed liquid 8.4m 3 , put the material liquid on the strong acid cationic resin column again, when it is detected that the resin is saturated, stop feeding, and use 0.8% ammonia water to elute the resin column, after passing the detection, collect the higher L-histidine solution 2.3 m 3 , neutralized with hydrochloric acid to pH = 5.8, added activated carbon for decolorization, filtered, exchanged the filtrate with a strong basic anion resin column, collected the resi...

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PUM

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Abstract

The invention discloses an L-histidine production method, the mixed amino-acid mother liquor that is acquired through a protein hydrolysis method is separated by secondary strong acid cationic resin column, neutralized by hydrochloric acid, decolorized by activated carbon, exchanged by strong alkali cationic resin column and crystallized, thus acquiring the product. The optical rotation of product acquired by the invention is as much as (Alpha)D20+12.1 degree-+12.8 degree, and the content is as much as 99.8-101.5 percent which even meets and exceeds the national standard of AJI97, and the product yield is as high as 1.2kg/m3 hydrolysate.

Description

technical field [0001] The invention relates to a production method of L-histidine, in particular to a resin separation method. Background technique [0002] The product form of L-histidine is white powder crystal. It is an important amino acid API, widely used in medical infusion products and health care products. [0003] At present, domestic L-histidine products can only rely on imports. Domestic research on L-histidine production technology is in the process of exploratory research, and the relevant content is limited to the literature. Tang Zongfang, Department of Biology, Department of Amino Acids, Wuhan University's paper "Systematic Separation of L-Valine, L-Leucine, L-Histidine, and L-Arginine from Pig Blood Meal", the analysis of histidine hydrochloride The production process has been studied. The method proposed in this document consumes a large amount of alcohol when obtaining histidine hydrochloride. In addition, there is also the problem that the purity of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64
Inventor 王先兵曾庆群肖国安章平吕德祥
Owner 湖北新生源生物工程有限公司
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