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Non-asepsis seedling re-cutting seedling-producing method of hippeastrum

A technology of aseptic seedlings and re-cutting, which is applied in the field of garden plants and ornamental horticulture, can solve the problems of factoryization, large-scale production, low efficiency, and long cycle, so as to reduce production costs, simplify processes, and promote growth. Effect

Inactive Publication Date: 2008-02-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing induction culture method for Hippeastrum seedlings has a long period, low efficiency, and relatively high cost, which cannot meet the requirements of industrialization and large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Obtaining of aseptic seedlings: select the bulbs of the excellent Hippeastrum variety Apple Blossom for tissue culture, first peel off the old scales of the outer layer and the old roots at the bottom, wash them with clean water, and cut off the top 2 / 3 scales. The scales at the base and the base plate are divided into 8-16 segments. Disinfect and inoculate after washing with soapy water and clean water respectively.

[0018] The medium is 1 / 2MS+BA2mg / L+NAA1mg / L, pH5.8, sugar 30g / L, AC2g / L, agar 6g / L. The culture conditions are: temperature 25°C, light: 16h light period / 8h dark period. A sterile vaccine was produced one month later.

[0019] 2. Cutting of sterile seedlings: when the diameter of sterile seedling bulbs reaches 3-5 mm, use the obtained small bulbs of sterile seedlings as materials to cut, and continue to induce culture for the cut sterile seedlings. The induction medium is: 1 / 2MS+BA4mg / L+NAA1mg / L+KH 2 PO 4 50mg / L+triiodobenzoic acid 0.5mg / L, adjust...

Embodiment 2

[0024] 1, the acquisition of aseptic seedlings: select the bulb of good Hippeastrum variety Vera (Vera) to carry out tissue culture,

[0025] The medium is 1 / 2MS+BA2mg / L+NAA1mg / L, pH5.8, sugar 30g / L, AC2g / L, agar 6g / L. The culture conditions are: temperature 25°C, light: 16h light period / 8h dark period. A sterile vaccine was produced one month later.

[0026] 2. Cutting of aseptic seedlings: cut when the diameter of the obtained aseptic seedling bulbs reaches 3-5 mm, and continue to induce culture of the cut aseptic seedlings. The induction medium is: 1 / 2MS+BA4mg / L+NAA1mg / L+KH 2 PO 4 60mg / L+triiodobenzoic acid 0.5mg / L, pH6.8, sucrose 40g / L, AC2g / L, agar 8g / L; culture conditions: temperature 20~30℃, light: 16h light period / 8h dark period; Obtain sterile seedlings with a length of 1 cm;

[0027] 3. Strong seedlings: For sterile seedlings with a length of 1cm, carry out strong seedling cultivation, and the medium is 1 / 2MS+BA4mg / L+NAA1mg / L+KH 2 PO 4 80mg / L+TIBA0.5mg / L, pH6...

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Abstract

The invention discloses a recutting method of sterile Hippeastrum seed in the garden plant and ornamental plant domain, which is characterized by the following: adopting the sterile seed through tissue culture as explant; cutting into small bulbs; culturing; breeding; obtaining offsprings continuously; adjusting pH value of the culture medium; adding plant growing inhibitor to improve the content of phosphor and potassium; accelerating the inducing rate and thick seed; improving the growing and breeding speed of Hippeastrum; adopting half MS culture medium to replace MS culture medium to reduce the cost; improving the survival rate of seed through two-step seed-transplanting pattern.

Description

technical field [0001] The invention relates to a method for cutting and forming seedlings of aseptic seedlings of Hippeastrum chinensis, which improves the reproduction and growth rate of tissue-cultured seedlings of Hippeastrum chinensis, and belongs to the field of garden plants and ornamental horticulture in agricultural biotechnology. Background technique [0002] Hippeastrum (Hippeastrum), also known as Amaryllis, Huazhoulan, Agapanthus, etc., is a perennial herbaceous plant of the genus Amaryllis in the family Amaryllidaceae. It is native to Peru and Brazil and is widely used as an ornamental bulb flower all over the world. During the period from 1912 to 1949 in our country, there were potted plants of Hippeastrum in Nanjing, Shanghai and other places. However, it is difficult to breed high-grade Hippeastrum, and my country’s Hippeastrum bulbs have always relied on imports from the Netherlands and other countries. At present, tissue culture or bulb cutting propagatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 史益敏邵素娟叶露
Owner SHANGHAI JIAO TONG UNIV
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