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Immune trait associated protein of pig, encoding gene and application of the same

A technology of related proteins and coding genes, applied in the direction of animal/human peptides, peptide sources, etc., can solve problems such as pigs that have not been reported

Inactive Publication Date: 2010-06-02
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the LBP genes of humans, mice, cattle, and chickens have been cloned, but the study of pig LBP has not been reported yet.

Method used

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  • Immune trait associated protein of pig, encoding gene and application of the same
  • Immune trait associated protein of pig, encoding gene and application of the same
  • Immune trait associated protein of pig, encoding gene and application of the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the acquisition of porcine immune traits-related protein (LBP) and its full-length coding region and partial genome sequence (pig LBP gene)

[0041] 1. Acquisition of porcine immune trait-related protein (LBP) and its full-length coding region

[0042] Using human LBP gene cDNA (GenBank accession number: NM_006263) as an information probe, using the BLAST tool in NCBI to perform homologous sequence screening in the GenBank pig EST database, to obtain a series of ESTs with a homology of more than 80% (the fragment length is greater than 100bp), then use the seqman program in DNAstar software to perform EST splicing sequence, and design primers according to the splicing sequence. The primer sequences are as follows:

[0043] F1: 5'-CTGCATCGGACCTGACCCA-3' (sequence 4 in the sequence listing)

[0044] R1: 5'-TGCCCTTCAAGAAAGTACCAGC-3' (sequence 5 in the sequence listing)

[0045] Extract the total RNA from the liver tissue of a Tongcheng pig, reverse transcr...

Embodiment 2

[0051] Example 2, Establishment of PCR-RFLP Diagnostic Method for Pig LBP Gene and Correlation Analysis of the Gene Marker Characters

[0052] 1. Establishment of PCR-RFLP diagnostic method for pig LBP gene

[0053] The partial genome gene sequence of pig LBP obtained in step 2 of Example 1 shows that the 191st nucleotide at the 5' end of sequence 3 in the sequence listing is a polymorphic site, which is A or G. When the 191st nucleotide at the 5' end of sequence 3 in the sequence listing is A, the gene is assumed to be controlled by the A allele; when the 191st nucleotide at the 5' end of sequence 3 in the sequence listing is G , the gene is assumed to be controlled by the B allele, and these two alleles can form three genotypes: pure and AA, BB, and hybrid AB.

[0054] When the 191st nucleotide at the 5' end of sequence 3 in the sequence listing is G, there is one MspI restriction site (C↓CGG), so the part of the pig LBP obtained by amplifying step 2 of Example 1 Genomic g...

Embodiment 3

[0063] Embodiment 3, the distribution of pig LBP gene genotype in different pig breeds

[0064] Different kinds of pigs (Wuzhishan pigs (34) shown in Table 1, Laiwu pigs (32), Tongcheng pigs (41), Landrace pigs (26), Large White pigs (26) were tested according to the method of Example 2. ), respectively purchased from the Wuzhishan Miniature Pig Breeding Farm of the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences; the Laiwu Black Pig Breeding Farm of Laiwu City, Shandong Province; to analyze

[0065] The results are shown in Table 3. According to the results of the genotype and gene frequency in Table 3, the B allele is dominant in the three domestic varieties of Wuzhishan, Tongcheng and Laiwu tested; while in the foreign varieties Changbai and Dabai The A allele is dominant.

[0066] Table 3 Distribution of LBP 329G / A mutation Msp I polymorphism in 5 pig breeds

[0067]

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Abstract

The invention discloses a pig immunity relative protein, coding gene and appliance, which is characterized by the following: possessing one of amino acid residue sequence as 1) SEQ ID N: 1 in sequenceand 2); replacing, deleting or adding the relative protein with pig immunity for SEQ ID N: 1 amino acid residue sequence in the sequence table through one to ten amino acid residues. This invention can be used to control pig immunity protein and gene, which can possesses important action in pig breeding.

Description

technical field [0001] The invention relates to a protein related to porcine immune traits and its coding gene and application, in particular to a protein related to porcine immune traits and its coding gene and a method for detecting pig immune performance by using the single nucleotide polymorphism of the protein coding gene. Background technique [0002] Animal immunity is directly reflected in disease resistance, which is closely related to the production capacity of animals. Healthy livestock and poultry have high production performance and low breeding management costs. Unhealthy livestock and poultry have low production performance and high breeding management costs. With the expansion of factory farming scale and the increase of breeding density, the probability of disease of livestock and poultry has increased greatly. On the other hand, severe infectious diseases have occurred frequently in recent years, and some mutated strains have strong pathogenicity, and som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47
Inventor 李奎刘华珍李新云唐中林杨述林崔文涛
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI