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Continuous bottling and culturing device of plant cell stirring type bioreactor

A bioreactor and plant cell technology, which is applied in the field of plant cell stirring bioreactor continuous perfusion culture device, can solve the problems of poor cell sedimentation effect and cell loss, so as to improve the sedimentation efficiency, reduce pollution, and increase the cross-section area effect

Inactive Publication Date: 2008-02-27
上海交通大学中原研究院
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  • Abstract
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Problems solved by technology

But its flaws and shortcomings are: when the laboratory repeated the experiment, it was found that the cell sedimentation effect was not good, and when the cell concentration was high, a large number of cells were lost in the waste liquid

Method used

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  • Continuous bottling and culturing device of plant cell stirring type bioreactor
  • Continuous bottling and culturing device of plant cell stirring type bioreactor
  • Continuous bottling and culturing device of plant cell stirring type bioreactor

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Embodiment Construction

[0027] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings: this embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following the described embodiment.

[0028] As shown in Figure 1, this embodiment includes: reaction tank 5, liquid storage bottle 1, sampling device, on-line monitoring system, gas supply device, magnetic stirrer 9, settling device, waste liquid bottle 12, and settling device also includes sequentially serial The primary settling device 15, the secondary settling device 10, and the tertiary settling device 11 are connected in rows. The liquid storage bottle 1, the sampling device, the on-line monitoring system, the gas supply device and the settling device are respectively connected with the reaction ta...

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Abstract

The invention discloses a continuous pouring culture device of plant cell stirring typed biological reactor in the cell engineering technical domain, which comprises the following parts: one-grade sediment device, two-grade sediment device, three-grade sediment device, liquor storage bottle, sampling device, on-line monitor system, gas supplying device, sediment device in connection with the reacting tank on the magnetic stirrer, wherein the waste liquor bottle connects the sediment device. The invention is a group of three-gravity inner-outer cylinder biological reactor of sediment cell interception device to provide reference to culture large scale of plant cell, which improves the separating efficiency of the cell and culture liquid of reactor to reduce the probability of pollution.

Description

technical field [0001] The invention relates to a device in the technical field of cell engineering, in particular to a plant cell stirring bioreactor continuous perfusion culture device. Background technique [0002] The development of plant natural products has always been a research hotspot. The use of bioreactors for plant cell suspension culture can not only produce many valuable secondary metabolites, but also alleviate the over-harvesting of wild resources. However, the phenolic substances produced by plant cells during the culture process are not conducive to cell proliferation, and cause cell browning and death in severe cases; in addition, the accumulation of secondary metabolites in the culture medium will inhibit the further synthesis of products. The continuous perfusion culture of plant cells can continuously bring the substances released by the cells into the medium to the outside of the reactor, effectively solving the problems of cell browning and product fe...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/02
CPCC12M27/02C12M23/58C12M33/22C12M29/10C12M21/02C12M29/06
Inventor 王贵荣齐念民张宝红闫继红廖凯
Owner 上海交通大学中原研究院
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