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Method for extracting vinca alkaloids extract from Vinca rosea

A technology of vinblastine and periwinkle, applied in the field of extracting vinblastine from periwinkle, can solve the problem of poor comprehensive utilization of resources, and achieve the effect of reducing emissions and improving utilization

Inactive Publication Date: 2008-04-09
刘全胜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic production of vinca total alkaloids only extracts vinblastine with a low content from vinca plants, while a large amount of monoindole alkaloids, vindolin and vinblastine, are discarded as waste, resulting in poor comprehensive utilization of resources

Method used

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with specific embodiments, so as to help understand the contents of the present invention.

[0013] The acid water below uses 2% H 2 SO 4 .

[0014] 1. Extraction of total alkali

[0015] Weigh about 4 kg of periwinkle hay, add a small amount of acid water to mix it wet, and then grind it with a homogenizer. Soak the crushed grass dregs in 8 liters of acid water for 24 hours. At this time, the pH of the acid water soaking solution is 1-1.5. Thereafter, the acidic aqueous solution was collected by filtration, adjusted to pH=7.5-9.0 with NaOH, the alkaline aqueous phase was extracted with chloroform, and the chloroform phase was collected and evaporated to dryness with a rotary evaporator at 30-50°C to recover the solid recovery. After the above operation is repeated three times, basically all the alkaloids in the periwinkle can be extracted. Then, the recovered product is dissolved in acid water an...

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Abstract

The invention relates to a method to extract the vinblastine extracted from the catharanthus roseus, including the following processes: (1) the catharanthus roseus is agitated and smashed after the wetting by the acidic water and then is soaked in the acidic water, the filtrate after the filtration is regulated to alkalinity by using alkali, a alkaline water phase is extracted by using chloroform, a chloroform phase is collected, and the solid reclaimed materials can be obtained after the reclaiming of the chloroform; (2) the reclaimed materials are dissolved by using the acidic water, the ethyl acetate is added for extraction, and a acidic water phase is reclaimed; (3) the reclaimed acidic water phase in step (2) is regulated to alkalinity, the chloroform is used for extraction, and the crude alkaloid is obtained by the reclaim of chloroform of the extraction phase; (4) the obtained crude alkaloid in step (3) is dissolved by using the acidic water, of which the pH is regulated to alkalinity, the lower layer of sediments is collected centrifugally, the refined catharanthus roseus alkaloid is obtained after the drying of the sediments, and the vinblastine and leurocristine can be obtained by alumina column chromatography separation; (5) the filtrate which is obtained by centrifugation in step (4) is extracted by using chloroform, the catharanthine and the vindoline are obtained by vacuum concentration and macroporous resin separation. The method can improve the utilization ratio of catharanthus roseus.

Description

technical field [0001] The invention relates to a new process for extracting vinblastine, wenduoling, vinblastine and vincristine from periwinkle flowers. Vinblastine and wenduoling are synthetic antitumor drugs vinorelbine, vinblastine and vincristine Important raw materials, vinblastine and vincristine can be directly used as medicine. Background technique [0002] Vinblastine antineoplastic drugs are bisindole alkaloids with anticancer activity and their structurally modified derivatives, which are isolated from Apocynaceae periwinkle. The natural ones are vinblastine and vincristine. Vinblastine is used to treat various solid tumors, and vincristine is mainly used to treat acute leukemia in children. Vinblastine and Wenduolin can be used to synthesize vinblastine, and further synthesize vinorelbine for the treatment of non-small cell lung cancer and advanced breast cancer. [0003] The content of bis-indole alkaloid vinblastine in vinca is about 1 / 100,000, and that of...

Claims

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Application Information

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IPC IPC(8): C07D519/04C07D461/00A61K36/24
Inventor 刘全胜
Owner 刘全胜
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