Enzyme catalysis tetrabutyl ammonium bromide syntony scattering spectrometry detecting trace amount H2O2
A technology of tetrabutylammonium bromide and H2O2, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex instruments, difficult to obtain reagents, and low sensitivity, and achieve simple equipment, simple and fast determination, and good selectivity Effect
Inactive Publication Date: 2008-04-09
GUANGXI NORMAL UNIV
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Among these methods, some have low sensitivity, some reagents are not easy to obtain, and some instruments are complicated.
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[0036] The contents of the present invention will be further elaborated below in conjunction with the accompanying drawings and examples, which describe that in weakly acidic HAC-NaAC buffer, when there is H 2 o 2 When present, HRP catalyzes H 2 o 2 Generate OH, OH oxidizes I - Generate I 2 , excess I - with I 2 combined to form I 3 - , while TBA + with I 3 - Binding formation (TBA-I 3 ) n The particles are associated, resulting in resonant scattering. Based on this, a resonance scattering spectroscopic analysis method for the indirect determination of hydrogen peroxide was established. Its main responses are as described above:
[0037]A Tetrabutylammonium Bromide Resonance Scattering Spectrometric Method for Detection of Trace H 2 o 2 method, including the following steps:
[0038] 1. In five 5mL graduated test tubes with stoppers, add HAc-NaAc buffer solution with a pH of 4.6 and a volume of 0.3ml, 0.2mL 0.04mol / L KI, and then add 0.8-0.02mL 0.2μg / L mL of ...
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Abstract
The invention discloses a resonance scattering spectrometric for detecting and measuring the enzyme catalysis tetrabutylammonium bromide of H2O2. The invention is characterized in that in the acidic condition, the horse radish peroxidase (HRP) can catalyze H2O2, and reacts with the excessive I- to form an I3-, the I3- reacts with tetrabutylammonium bromide (TBA) to form an associated matter particulate (TBA-I3)n, the associated matter particulate has the resonant scattering effect. The concentration of the H2O2 presents a good linear relation with the resonant scattering strength of the TBA system, and the method for detecting and measuring H2O2 is established according to the good linear relation, and the method has the advantages that the sensitivity is high, the selectivity is good and simple and quick. The invention has the advantages that the device is simple, the operation which can be completed with a fluorospectrophotometer is convenient; the reagent is easy to obtain, the cost is low; the substrate-potassium iodide is innocuous; the sensitivity is high, the detection limit is 43.7nmol / l, the speed is high, the reaction only needs 35 minutes, and the invention has a better application value.
Description
Technical field: [0001] The present invention relates to H 2 o 2 detection method, especially a detection method for trace H 2 o 2 Enzyme-Catalyzed Tetrabutylammonium Bromide Resonance Scattering Spectroscopy. Background technique: [0002] h 2 o 2 Commonly known as hydrogen peroxide, it is a colorless and heavy liquid with strong oxidizing and corrosive properties. It can be used as a disinfectant, dechlorination agent, bleaching agent, oxidizing agent, and for rocket fuel, organic or inorganic peroxides, foam plastics and other Porous substances, etc., are widely used in medicine, national defense, industry, agriculture, chemical industry and other fields. Since it is the product of many peroxidase-catalyzed reactions, in the fields of food science, pharmaceutical science and environmental science, it is necessary to establish a sensitive, fast and easy detection of H 2 o 2 method is particularly important. Currently, the determination of H 2 o 2 The methods mai...
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IPC IPC(8): G01N33/541G01N21/63
Inventor 马纪蒋治良
Owner GUANGXI NORMAL UNIV