Kit for quantitative determining enrofloxacin content in food product and testing method thereof

A technology for quantitative detection of enrofloxacin, applied in the field of immunoanalysis, can solve the problems of on-site rapid detection of unsuitable samples, expensive equipment, time-consuming detection process, etc., achieve good broad-spectrum and sensitivity, low detection cost, easy operation easy effect

Inactive Publication Date: 2008-04-16
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

Among them, the most researched and applied at home and abroad is the HPLC method, but the pretreatment process of this method is cumbersome, the operation is complicated, and usually requ

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  • Kit for quantitative determining enrofloxacin content in food product and testing method thereof
  • Kit for quantitative determining enrofloxacin content in food product and testing method thereof

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Embodiment Construction

[0036] The present invention is further described in detail by the following examples, which are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0037] The invention is a kit for quantitatively detecting the content of enrofloxacin in food, which consists of a coated plate and a reagent group, wherein the reagent group consists of:

[0038] (1) Buffer: phosphate buffer, pH 7.4, diluted 1:5 with secondary water when used;

[0039] (2) Substrate A: a sodium acetate buffer containing urea hydrogen peroxide;

[0040] (3) Substrate B: a dimethyl sulfoxide solution containing 3,3',5,5'-tetramethyl-p-diaminobenzidine TMB;

[0041] (4) Washing solution: phosphate buffer containing 2.5% Tween; dilute with secondary water according to 1:5 when used;

[0042] (5) Termination solution: 1.25mol / L sulfuric acid;

[0043] (6) Enrofloxacin standard solution: the concentration of the enrofloxacin standard solution...

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Abstract

The invention relates to a reagent kit for quantificationally detecting the content of enrofloxacin in foods, belonging to the immunoassay technical field, consisting of a covering plate and a reagent group, wherein, the reagent group comprises buffer solution (1), a zymolyte A (2), a zymolyte B (3), scrub solution (4), stop solution (5), enrofloxacin standard solution (6) and enzyme-marked enrofloxacin antigen solution (7); a millipore of the covering plate is covered with polyclone enrofloxacin antibody. The invention has the characteristics of simple manipulation, high detection sensitivity, strong specificity, good accuracy and low cost of detection. Moreover, the invention is of wide range of application. With economic and social benefits, the invention is a reagent kit for quantificationally detecting the content of enrofloxacin in foods with high creativity and good potential applicaiton.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a kit for quantitatively detecting the content of enrofloxacin in food and a detection method thereof. Background technique [0002] Enrofloxacin (Enrofloxacin) also Ming ethyl ciprofloxacin, the chemical structure is (C 19 h 22 FN 3 o 3 ), the molecular weight is 359.1646. Enrofloxacin can cause toxic side effects such as neurotoxicity, renal function damage, allergic reaction, and arthritis in young animals, and its residues in animal foods directly threaten human health. In addition to its direct harm to humans due to its toxic and side effects, what is more serious is that the low-concentration drug residues in animal foods can easily induce drug resistance in human pathogenic bacteria, which is not conducive to the treatment of human diseases by such drugs , so we must attach great importance to the residues of enrofloxacin in animal foods. In recent years, the...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/531
Inventor 王硕王忠斌张燕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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