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Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

A carbon dioxide and kit technology, which is applied in the field of carbon dioxide diagnosis/determination kits and determination of carbon dioxide concentration, which can solve problems such as lack of experimental research.

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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  • Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

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Experimental program
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Effect test

Embodiment 1

[0047] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0048] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0049] Stabilizer 50mmol / L

[0050] Reduced coenzyme 0.25mmol / L

[0051] Pyruvate dehydrogenase 10000U / L

[0052] Lactate dehydrogenase 16000U / L

[0053] Acetyl-CoA 6mmol / L

[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0055]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0056] After adding the ...

Embodiment 2

[0058] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0059] Reagent 1

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 50mmol / L

[0062] Reduced coenzyme 0.25mmol / L

[0063] Acetyl-CoA 9mmol / L

[0064] Reagent 2

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Pyruvate dehydrogenase 16000U / L

[0068] Lactate dehydrogenase 24000U / L

[0069] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0070] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (downward...

Embodiment 3

[0073] The carbon dioxide diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0074] Reagent 1

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 50mmol / L

[0077] Reduced coenzyme 0.25mmol / L

[0078] Reagent 2

[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0080] Stabilizer 50mmol / L

[0081] Acetyl-CoA 4mmol / L

[0082] Reagent 3

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 50mmol / L

[0085] Pyruvate dehydrogenase 20000U / L

[0086] Lactate dehydrogenase 36000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring carbon dioxide concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, tes...

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Abstract

The invention relates to a carbon dioxide diagnosing / testing reagent box which utilizes the enzyme multiplying method, the enzyme color method and the enzyme connection technology, and also relates to a method for testing the consistence of carbon dioxide, and the elements and the components of reagent. The invention belongs to the technical field of the medicine / food / environment check. The reagent box of the invention mainly comprises cushion fluid, reduced enzyme, acetyl A, pyruvate dehydrognase, dehydrogenase and stabilizer. A series of enzyme reactions occur by mixing the sample and the reagent according to a certain volume rate. Then the reactant is arranged under an ultraviolet and visible light analysis instrument to test the reduction extend and the speed of the absorbency at the 340nm of the main wavelength, thereby testing the consistence of the carbon dioxide. By adopting the invention, the testing result can be obtained by the ultraviolet and visible light analysis instrument, and the promotion and the application are convenient.

Description

technical field [0001] The invention relates to a carbon dioxide diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring carbon dioxide concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicar...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD