Method for improving arteannuin content in abrotanum with abscisic acid process
A technology of artemisinin and abscisic acid, which is applied in horticultural methods, botany equipment and methods, chemicals for biological control, etc., can solve the problem of low feasibility of artemisinin, and achieve the convenience of large-scale production, The effect of simple operation and reduced production cost
Inactive Publication Date: 2010-06-02
SUZHOU TANGJI BIOTECH CO LTD
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However, the content of artemisinin in the callus is less than 0.1% of the dry weight, and the highest in the shoot is only 0.16% of the dry weight, and most studies have not detected artemisinin in the root, so the use of tissue culture and cell Engineering to produce artemisinin is not feasible
In addition, using genetic engineering technology to obtain new varieties of Artemisia annua with higher content of artemisinin is a more feasible method, but the use of genetic engineering to increase the content of artemisinin is still in the experimental stage
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[0020] The seeds of Artemisia annua (derived from the Artemisia annua species with higher artemisinin content in Youyang, Chongqing) were planted on the seedling medium to sprout, and the formula of the seedling medium was MS culture fluid, which added 30g / L of sucrose and 2.6g / L plant gel. When the Artemisia annua seedlings grew to about 5 cm, they were transplanted into peat-perlite culture medium (the ratio of peat and perlite was 1:1), and watered once a week with ordinary nutrient solution. Wherein the common nutrient solution component weight percent is: 0.0033% NH 4 NO 3 , 0.0038% KNO 3 , 0.00088% CaCl 2 .2H 2 O, 0.00074% MgSO 4 .7H 2 O and 0.00034% KH 2 PO 4 , and the balance is water. When the plants grow to about 40-60cm (the early flowering stage of the plants), the Artemisia annua plants are treated with an abscisic acid solution, and the Artemisia annua plants treated with water are used as a control. Two days later, the leaves of the whole plant of Arte...
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The invention relates to an abscisic acid treatment method for enhancing the arteannuin content in the sweet wormwood, and belongs to the biological technology field. In the invention, a sweet wormwood seed is planted in the seedling culture medium to perform sprouting firstly, then the sweet wormwood sprout is transplanted into the turf pearlite culture medium, the sweet wormwood sprout is treated through abscisic acid solution two days before harvesting, and then the arteannuin content in the sweet wormwood can be enhanced. The invention can effectively enhance the arteannuin content in thesweet wormwood, the operation is simple, the comparatively higher initial investment required by the arteannuin content enhancing method through the gene engineering is not required, the arteannuin production cost is effectively reduced, and the invention has an important meaning to the large scale production of the arteannuin.
Description
technical field [0001] The invention relates to a method for increasing the content of artemisinin in the field of biotechnology, in particular to a method for increasing the content of artemisinin in Artemisia annua by treating with abscisic acid. Background technique [0002] Artemisinin has been recognized all over the world as an effective antimalarial drug. At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. Artemisia annua L. (Qinghao), also known as Artemisia annua, is an annual herb medicinal plant. Due to the very low content of artemisinin in Artemisia annua (0.01%-1% dry weight), the large-scale commercial production of this drug is limited. Although artemisinin can be artificially synthesized now, it is not feasible for commercial production due to the difficulty, low yield and high cost. [0003] The use of tissue culture and cell engineering to produce artemisinin is an attractive approach. However, the c...
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IPC IPC(8): A01G31/00A01G7/00A01P21/00A01G1/00A01N37/00
Inventor 唐克轩景福远张凌王国丰
Owner SUZHOU TANGJI BIOTECH CO LTD