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Method for preparing folium ginkgo extract from Hericium erinaceus and usage

A technology of Hericium erinaceus and Ginkgo biloba is applied in the field of biotransformation to achieve the effects of easy management, saving manpower and material resources, and low cost

Inactive Publication Date: 2008-05-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research on Hericium erinaceus mainly focuses on the submerged fermentation and mycelium of Hericium erinaceus. The research on Ginkgo biloba extract focuses on the preparation and preparation method of the extract components. For Hericium erinaceus, the Ginkgo leaf extract is transformed to obtain its fermentation liquid For the development of hypoglycemic drugs, there is no patent report in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Hericium erinaceus is inoculated into the bran solid medium, cultured for 3-7 days respectively, and then transferred into the seed medium. After the seeds are cultured at 25-28°C and 150r / min for 1-2 days, they are transferred to the supplemented fermentation medium for culture. Medium composition: glucose 40g / L, peptone 10g / L, bran 5g / L, corn flour 20g / L, MgSO 4 ·7H 2 O 1g / L, KH 2 PO 4 2 g / L, EGB 5g / L and 10g / L CaCO 3 . Fermentation process: the conversion temperature is 30°C, and the initial pH range is 7.5. The fermentation culture conditions are as follows: the ventilation rate is 2:1, the rotation speed is 150 rpm, and the temperature is 35°C.

[0011] After the fermented liquid was prepared, the hypoglycemic experiment of diabetic rats was carried out. Normal male SD rats were adaptively fed for 5 days before the experiment, and 10 rats were selected as the normal control group. After the other rats were fasted for 24 hours, they were intraperitoneally inj...

Embodiment 2

[0018] Hericium erinaceus inoculation was the same as in Example 1 through bran solid medium and seed medium cultivation. After culturing for 1-2 days, transfer to supplemented fermentation medium for culturing. Composition of fermentation medium: glucose 10g / L, peptone 2g / L, bran 1g / L, corn flour 5g / L, KH 2 PO 4 0.1g / L, 0.5% EGB and 1g / L CaCO 3 . The fermentation culture conditions are as follows: the ventilation rate is 0.1:1, the rotation speed is 30 rpm, and the temperature is 20°C.

[0019] After the fermented liquid was prepared, the hypoglycemic experiment of diabetic rats was carried out. Normal male SD rats were adaptively fed for 5 days before the experiment, and 10 rats were selected as the normal control group. After the rest of the rats were fasted for 24 hours, they were intraperitoneally injected with 200 mg / kg alloxan to make the model. After 5 days, they were fasted for 3-5 hours, and their blood sugar was measured. The rats with a blood sugar value > 11 m...

Embodiment 3

[0023] Hericium erinaceus inoculation was the same as in Example 1 through bran solid medium and seed medium cultivation. After the seeds are cultivated for 1-2 days, they are transferred to the fermentation medium for cultivation. Medium composition: glucose 25g / L, peptone 6g / L, bran 3g / L, corn flour 12.5g / L, MgSO 4 ·7H 2 O 0.5g / L and KH 2 PO 4 1g / L, 2.5% EGB and 5g / L CaCO 3 . The fermentation culture conditions are as follows: the ventilation rate is 1:1, the rotation speed is 90 rpm, and the temperature is 28°C.

[0024] After the fermented liquid was prepared, the hypoglycemic experiment of diabetic rats was carried out. Normal male SD rats were adaptively fed for 5 days before the experiment, and 10 rats were selected as the normal control group. After the rest of the rats were fasted for 24 hours, they were intraperitoneally injected with 200 mg / kg alloxan to make the model. After 5 days, they were fasted for 3-5 hours, and their blood sugar was measured. The rats...

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Abstract

The invention pertains to the field of biological transformation technology, which relates to a preparation method and the usage of fermentation liquid and fermentation liquid extract of ginkgo biloba leaf transformed by hericium erinaceus. The invention inoculates the hericium erinaceus in a wheat bran solid culture medium to be cultured respectively for 3 to 7 days, after that, the mixture is firstly trans-inoculated in a seed culture medium to be cultured for 1 to 2 days and is finally trans-inoculated in a fermentation culture medium to be cultured, wherein, the seed culture medium composition is: 10 to 40g / L of glucose, 1 to 5g / L of wheat bran, 2 to 10g / L of peptone and 5 to 20g / L of corn flour; the fermentation culture medium is: 10 to 40g / L of glucose, 1 to 5g / L of wheat bran, 2 to 10g / L of peptone, 5 to 20g / L of corn flour, 0 to 1g / L of MgSO4.7H2O, 0.1 to 2g / L of KH2PO4, 1 to 10g / L of CaCO3 and 0.2 to 5 percent EGB. The method of the invention has low cost, simple process, convenient management and can save manpower and material resources; furthermore, the tests for rats with diabetes prove that the prepared fermentation liquid can be used in the preparation of the drugs for lowering blood glucose.

Description

Technical field: [0001] The invention belongs to the technical field of biotransformation, relates to biotransformation and hypoglycemic effect, and specifically relates to the preparation method and application of the fermented liquid and fermented liquid extract of Hericium erinaceus transforming ginkgo leaves. Background technique: [0002] Hericium erinaceus (Hericium erinaceus) is a well-known edible and medicinal fungus. It is flat in nature and sweet in taste, and has the functions of helping digestion and benefiting the five internal organs. Studies have shown that Hericium erinaceus can improve the body's ability to resist hypoxia, increase cardiac output, accelerate blood circulation, and inhibit the growth of tumor cells, etc., especially has a good protective effect on gastric mucosa. [0003] The biotransformation and modification of some natural products with known structures that have medicinal effects is a hot spot in current research. At the same time, it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P3/10C12N1/14
Inventor 黄达明张志才钱静亚崔凤杰肖香管国强吴其飞
Owner JIANGSU UNIV
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