Method of í«hanfuí» apple anther culture plant strain

Abstract

The invention relates to a plant cultivation method of a Hanfu apple anther, pertaining to the agricultural field. The invention comprises a Hanfu apple anther acquisition and disinfection and cleaning process 1, an embryoid abduction process 2, a plant differentiation process 3 and an abduction rootage and test tube plantlet grafting process 4. Compared with the prior art, the method of the invention has the advantages that: (1) such expensive hormones as 2,4-D, IAA, K, BA are reduced for an embryoid induction culture medium, thus reducing the cost and shortening the embryoid induction period by 60 days; (2) a novel hormone TDZ with comparatively better activity is adopted for an embryoid differentiation culture medium, thus improving the differentiation rate, and the differentiated plants grow well, which shows that the invention is beneficial to the rootage and grafting; (3) the plant can be grafted to the twig of greenhouse potted malus bacata in March, to enhance the survival ratio and greatly lengthen the growth cycle, thus reaching complete lignification and safely hibernating. The method of the invention is suitable for cultivating apple plants with apple anther.

Description

technical field [0001] The invention relates to a method for raising seedlings of plants in the field of agriculture, in particular to a method for cultivating Hanfu apple anthers. Background technique [0002] Apple is a cross-pollinated plant with a high degree of heterozygosity in genetics. It is very difficult to obtain pure line materials. It is relatively easy to obtain haploid plants through anther culture, and then obtain pure line materials through chromosome doubling. This technology has very important significance in the research of apple breeding. [0003] The Fruit Tree Research Institute of the Chinese Academy of Agricultural Sciences is the earliest experimental research institution in my country to use apple anthers to cultivate haploid plants. Tissue culture of anthers was successful. [0004] In related papers, the method of utilizing apple anther tissue culture is announced, the content of which is: the medium for inducing embryoid bodies is MS supplement...

AI Technical Summary

Problems solved by technology

[0011] Because the medium contains 2,4D hormones, although it is beneficial for apple anthers to induce embryoid bodies, it affects the later growth and development of plants

Due to the special anther biological characteristics of Hanfu apple varieties, the currently published methods for cultivating apple anthers to obtain plants are difficult to meet the needs of Hanfu apple anther culture plants.