Method for synchronously producing hypocrellin and shiraia bambusicola polysaccharides

Abstract

The invention relates to the field of bio-fermentation, in particular to a method of producing hypocrellin pigments and polysaccharides simultaneously with solution fermentation. In the art of producing hypocrellin pigments and polysaccharides simultaneously with solution fermentation of the invention, bottle-shaking culture is firstly proceeded, convolution culture is the second step, the enlarging culture of the shaking bottle is the third step and fermentation culture is the final step. In the step of bottle-shaking culture, strain of the hypocrellin is utilized as parent strain, bacteria on the inclined surface of the tube are introduced to a shaking bottle inside which liquid medium is arranged, and the charging coefficient of the shaking bottle is 0.1 to 0.25. In the step of convolution culture, the rotation speed of the convolution culture is 150 to 250 turnings every minute and the culture temperature is 22 to 27 DEG C. In the third step of enlarging culture of the shaking bottle, the culture material achieved by the bottle-shaking culture is shifted to the culture medium of the enlarging culture of the shaking bottle to proceed culture, the inoculation amount is 2-10%, the rotation speed of a rotation bed is 150-250 turnings every minute, the culture temperature is 22 to 27 DEG C, and the culture period is 72 to 120 hours. In the final step of fermentation culture, the culture material achieved by the enlarging culture of the shaking bottle is transferred to a fermentation pot inside which fermentation culture medium is installed to be cultured, the inoculation amount is 5-10%, and the culture temperature is 22 to 27 DEG C. The hypocrellin fermentation liquid is achieved after the fermentation is finished, and the hypocrellin pigments and polysaccharides are respectively achieved by separating and purifying the achieved fermentation liquid.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for simultaneous production of hypocretin and bamboo yellow polysaccharide by a liquid fermentation method. Background technique [0002] Bamboo yellow fungus (Shiraia bambusicola Henn.) is also known as Chituanzi, bamboo red dumpling, bamboo cocoon, bamboo red spot fungus, light chrysanthemum, Tianzhu flower, light bamboo flower, bamboo flower, etc., and is a fungus of the family Hypocreaceae. The fruiting bodies (also known as sub-seats) formed on specific bamboos. It is warm in nature and light in taste, and has the effects of relieving cough and pain, relaxing tendons and activating collaterals, dispelling wind and dampness, invigorating qi, invigorating blood and enriching blood, dissipating blood stasis and dredging menstrual flow. Bamboo yellow is used in the folks to treat diseases such as deficiency and cold stomachache, rheumatoid arthritis, tracheitis, ...

AI Technical Summary

Problems solved by technology

Both hypocrellin and bamboo yellow polysaccharide are biologically active substances with good application prospects. The simultaneous production of hypocrellin and bamboo yellow polysaccharide by liquid fermentation can obtain these two substances at the same time, but there is no relevant research on the use of liquid fermentation method. Research Report on Simultaneous Production of Hypocretin and Phyllostachys Polysaccharide