Method for abstracting and purifying betulin in birch bark

A technology of betulin and birch bark, applied in the field of phytochemistry, can solve the problems of only 1.7% yield, high production cost, cumbersome process and the like, and achieves the effects of easy recycling, saving extraction time and simple process

Active Publication Date: 2008-06-18
CHINA GATEWAY PHARMA DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the process of bark crushing is loaded down with trivial details, and equipment investment is big, and production cost is high
[0006] (3) Patent CN 1634972 uses ethyl acetate to extract crushed white birch bark, then concentrates under reduced pressure, filters after standing, collects the precipitate, adds activated carbon to the precipitate and extracts it with methanol reflux, recovers methanol under reduced pressure, and collects betulin , its purity is greater than 80%, but the yield is only 1.7%
The method is cumbersome and costly
In addition, the amount of filler required for traditional chromatography column packing is large, generally 10 to 100 times the weight of the sample
Therefore, the traditional chromatography method is not suitable for the large-scale extraction and purification of betulin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) 1kg of birch bark is chopped into bark particles with a particle diameter of 10mm with a Chinese medicine raw material with a granulator.

[0027] (2) Put the bark particles into 95% ethanol aqueous solution by volume and heat and reflux to extract 4 times, extracting for 2 hours each time, the weight-to-volume ratio of the bark particles to the aqueous alcohol solution for extraction is 1g: 12mL, and filter.

[0028] (3) The filtrate is concentrated under reduced pressure to the original volume of 0.5, the concentrated solution is allowed to stand and cooled to room temperature, and the precipitated crude betulin alcohol is collected, the upper layer is further concentrated to the original volume of 0.5, the concentrated solution is allowed to stand and cooled to room temperature, and the precipitated crude betulin is collected Fatty alcohol, combine the crude betulin that separates out twice; Wash crude betulin twice with 20% ethanol aqueous solution by volume perc...

Embodiment 2

[0033] (1) 1kg of birch bark is chopped into bark particles with a particle diameter of 4mm with a Chinese medicine raw material with a granulator.

[0034] (2) Put the bark particles into 85% ethanol aqueous solution by volume and heat and reflux for extraction 5 times, each extraction for 1.5 hours, the weight-to-volume ratio of the bark particles to the ethanol aqueous solution is 1g: 10mL, and filter.

[0035] (3) The filtrate is concentrated under reduced pressure to the original volume of 0.2, left to stand and cooled to room temperature, and the precipitated crude betulin is collected, and the upper layer is further concentrated to the original volume of 0.4, left to cool to room temperature, and the precipitated crude betulin is collected, combined The crude betulin separated out twice; the crude betulin was washed twice with 30% ethanol aqueous solution and dried in the sun to obtain 240 g of crude betulin.

[0036] (4) For crude betulin, add 5 times (weight to volume...

Embodiment 3

[0040] (1) 1 kg of birch bark is chopped into bark particles with a particle diameter of 2 mm with the raw material of traditional Chinese medicine with a granulator.

[0041] (2) Put the bark particles into 70% ethanol aqueous solution by volume and heat and reflux to extract 2 times, extracting for 2 hours each time, the weight-to-volume ratio of the bark particles to the ethanol aqueous solution is 1g: 12mL, and filter.

[0042] (3) The filtrate is concentrated under reduced pressure to the original volume of 0.3, left standing and cooled to room temperature, and the precipitated crude betulin is collected, the upper layer is further concentrated to the original volume of 0.6, left to cool to room temperature, the precipitated crude betulin is collected, and combined Twice precipitated betulin; hot air drying to obtain 180 g of crude betulin.

[0043] (4) For crude betulin, add 1 times (weight to volume ratio) of methanol to partially dissolve it, add 0.2 times the weight o...

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Abstract

The invention relates to a method for extracting and purifying the betulin from birch bark. The invention comprises the following procedures, cutting the birch bark into the bark particles, heating, refluxing and extracting by aqueous ethanol solution, filtering, vacuum concentration of the filtrate, cooling, collecting and drying the separated crude betulin. The method of the invention can obtain the betulin with the purity of more than 90 percent, and has the advantages of simple process, lower cost and being suitable for the industrial production.

Description

technical field [0001] The invention relates to the field of phytochemistry, in particular to a method for extracting and purifying betulin from birch bark. Background technique [0002] Birch bark is the bark of birch, a deciduous tree in the Betaceae family, and is a by-product of the logging industry. It contains betulin, fatty acids, tannins, polysaccharides, saponins and a small amount of triterpenoids such as betulinic acid. Wherein, the content of betulin is 23%-33% by weight. Betulin has a variety of biological activities, such as anti-tumor, anti-inflammatory and anti-viral activities, and has great development value. Betulin derivatives synthesized from betulin have stronger and wider biological activities. [0003] So far, the existing separation and purification methods have the following types, but all have various defects and cannot meet the needs of production: [0004] (1) Patent RU 2270202 uses methyl tert-butyl ether (MTBE) to extract betulin, and MTBE ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K36/185A61K129/00
Inventor 陈伟李飞燕
Owner CHINA GATEWAY PHARMA DEV CO LTD
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