Methods of suppressing UV light-induced skin carcinogenesis

A technology for ultraviolet light and skin cancer, which is used in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc.

Inactive Publication Date: 2008-06-25
THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, whether sulforaphane protects against UV-induced carcinogenesis remains to be determined

Method used

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  • Methods of suppressing UV light-induced skin carcinogenesis
  • Methods of suppressing UV light-induced skin carcinogenesis
  • Methods of suppressing UV light-induced skin carcinogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1. Preparation of sulforaphane from broccoli sprouts

[0047]Seeds of cauliflower (Brassica oleracea italica, cv. DeCicco) certified to have not been treated with any pesticides or other seed treatment chemicals were germinated and treated as described by Fahey et al. (12). Briefly, seeds were surface disinfected with a 25% aqueous solution of Clorox(R) bleach containing a trace amount of ALconox(R) detergent and rinsed thoroughly with water. The seeds were then spread in a single layer in inclined perforated plastic trays and misted with filtered water for 30 seconds, about 6 times per hour, with fluorescent lighting on. After 3 days, the sprouts were directly dropped into the boiling water in the steam-jacketed pot to make it stop growing, and the steam-jacketed pot was boiled again and stirred for about 5 minutes. This treatment inactivates endogenous myrosinase in shoots and extracts glucosinolates. The precursor glucoraphanin of sulforaphane was found to be the ...

Embodiment 2

Example 2. Treatment of Keratinocytes with Sulforaphane

[0048] Glutathione is the major and most abundant cellular nonprotein thiol and constitutes a key part of cellular defense: it readily reacts with potentially harmful electrophiles, participates in reactivity by scavenging free radicals and reducing peroxides Detoxification of oxygen intermediates and their toxic metabolites. The ability to increase cellular GSH levels is critical to combat oxidative stress. To this end, we investigated the ability of sulforaphane-induced phase 2 responses against UVA-induced oxidative stress in keratinocyte cultures. UVA was chosen for this study because its genotoxicity is thought to be mainly due to the generation of reactive oxygen intermediates.

cell culture

[0049] HaCaT human keratinocytes (gifted by G.Tim Bowden, Arizona Cancer Center, Tucson) were cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 5% FBS; PE mouse keratinocytes (Stuart H.Yuspa, National...

Embodiment 3

Example 3. Effect of Topical Administration of Sulforaphane on NQO1 and GSH in Mice

[0053] Phase 2 responses were next assessed in vivo in SKH-I hairless mice. Female SKH-I hairless mice (4 weeks old) were obtained from Charles River Breeding Laboratories (Wilmington, MA) and acclimatized in our animal facility for 2 weeks prior to the start of experiments. Animals were housed on a 12 hr light / 12 hr dark cycle, 35% humidity, with free access to water and pelleted AIN76A diet (Harlan TekLad, no inducer). All animal experiments were in accordance with National Institutes of Health Guidelines and were approved by the Johns Hopkins University Animal Care and Use Committee.

[0054] With 100 μl standardized myrosinase-hydrolyzed broccoli sprout extract (containing 1 μmol sulforaphane) or medium (100 μl 80% acetone: 20% water, v / v), 7-week-old SKH-I hairless Rats (5 per group) were treated locally on their backs. Animals were euthanized 24 hours later, and the dorsal skin was ...

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Abstract

ABSTRACT Administration of the isothiocyanate protects against UV light-induced skin carcinogenesis. In particular, topical application or dietary administration of isothiocyanate sulforaphane after exposure to UV radiation provides effective protection against skin tumor formation. Sulforaphane analogs and glucosinolates also can be employed. Lotions useful for suppressing UV light-induced skin carcinogenesis also are provided.

Description

Background of the invention [0001] The incidence of skin cancer has steadily increased to epidemic proportions: the average rate of increase in new skin cancer diagnoses has been 3-8% per year since the 1960s, and non-melanoma skin cancer is now the most common type of cancer in the United States, New cases exceed 1 million per year (1, 2). This steady increase in incidence is expected to continue, largely due to stratospheric ozone depletion, increased human exposure to solar radiation and longer average life expectancy. According to estimates by the National Cancer Institute, 40-50% of Americans over the age of 65 will develop at least one skin cancer and are at high risk for additional tumors (1, 2). Therefore, a detailed understanding of underlying risk factors and the development of new prevention strategies are urgently needed. [0002] It is widely accepted that UV radiation is the main cause of most non-melanoma skin cancers. Ultraviolet radiation is probably the mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/095A61P35/00
CPCA61K31/095A61P35/00A61K8/46
Inventor P·塔拉莱A·T·丁科瓦-科斯托瓦
Owner THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
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