Method for establishing rat hemorrhagic cerebral infarction animal model by collagenase revulsion

Abstract

The present invention relates to a method of establishing a rat bleeding cerebral infarction animal model by a collagenase inducement method, comprising the following steps: (1) the preparation of the rat, (2) preparing for a PE sleeve improved linear pin, (3) establishing a cerebral median occlusion model of a SD rat by a linear pin method which is making the model by inserting the linear pin into the artery outside the neck referred to Longa, Kuga etc., (4) pulling out a tube core and infusing the collagenase into the PE sleeve after the success of the cerebral median occlusion of the rat, (5) preparing for a reperfusion model and (6) putting a bleeding cerebral infarction rat model obtained by the steps back to a cage to breed continuously and processing for ethological grading towards the animal at different times after the operation respectively. The rat selected by the present invention is a grown male SD rat with low blood vessel aberrance rate, which improves the success rate of manufacturing the model. The bleeding quantity and the bleeding time of the established animal model can be controlled manually, and the operation wound is little, which does not influence the rat limb function and the ethological grading is exact, which has good stability and repeatability.

Description

[0001] The invention relates to a method for establishing an animal model of rat hemorrhagic cerebral infarction, which is used for simulating, researching, preventing and treating human hemorrhagic cerebral infarction and other medical fields. Background technique [0002] Cerebrovascular disease is a disease with high morbidity, high mortality and high disability rate, which seriously affects people's health and quality of life. Cerebrovascular disease ranks second in the statistics of the causes of death among Chinese nationals, accounting for about 14.85%. About 1.5 million patients die of cerebrovascular disease every year, and about 2 / 3 of the survivors are left with disabilities. About 70% of cerebrovascular diseases are ischemic stroke, and hemorrhagic cerebral infarction is a common complication of acute ischemic stroke, and the incidence of hemorrhagic cerebral infarction is reported at home and abroad to be about 3% to 43%. The incidence of hemorrhage after massive ...

AI Technical Summary

Problems solved by technology

The animal model of focal cerebral infarction was established by injecting autologous blood thrombus, and then the thrombolysis with urokinase was given through the mandibular vein at different time points, so as to establish the animal model of hemorrhagic cerebral infarction, which is more in line with the clinical occurrence of hemorrhagic cerebral infarction. Pathophysiological state, but due to the uncertainty of the infarction site and bleeding site, bleeding volume and time of the model, the stability and repeatability of the model are poor, which cannot meet the requirements of clinical application