Method for establishing rat hemorrhagic cerebral infarction animal model by collagenase revulsion

An animal model, collagenase technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of inability to meet clinical applications, model stability, poor repeatability, etc., and achieve accurate and stable behavioral scoring. and repeatability, and the effect of improving the success rate of modeling

Inactive Publication Date: 2008-07-09
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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Problems solved by technology

The animal model of focal cerebral infarction was established by injecting autologous blood thrombus, and then the thrombolysis with urokinase was given through the mandibular vein at different time points, so as to establish the animal model of hemorrhagic cerebral infarction, which is more in line with t

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Embodiment

[0021] The method for establishing the rat model of hemorrhagic cerebral infarction provided in this embodiment comprises the following steps:

[0022] (1) Preparation of rats: clean-grade male SD rats were selected and bred in an animal breeding laboratory for standby use. The weight of the rats is selected in the range of 300-320g. The distance from the beginning of the internal carotid artery to the opening of the middle cerebral artery in rats in this weight range is about 18.5±0.5mm, and it is easy for stereotaxic positioning. The animal level is clean to prevent the interference and influence of other pollution on the built model and improve the survival rate of the model. The rats were fed with standard feed and drank purified water, and the feeding environment was the multi-layer laminar flow frame of the Animal Experiment Center of our hospital.

[0023] (2) Preparation of PE casing improved thread bolt:

[0024] ①Materials used: PE pipe (outer diameter 0.38mm, inne...

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Abstract

The present invention relates to a method of establishing a rat bleeding cerebral infarction animal model by a collagenase inducement method, comprising the following steps: (1) the preparation of the rat, (2) preparing for a PE sleeve improved linear pin, (3) establishing a cerebral median occlusion model of a SD rat by a linear pin method which is making the model by inserting the linear pin into the artery outside the neck referred to Longa, Kuga etc., (4) pulling out a tube core and infusing the collagenase into the PE sleeve after the success of the cerebral median occlusion of the rat, (5) preparing for a reperfusion model and (6) putting a bleeding cerebral infarction rat model obtained by the steps back to a cage to breed continuously and processing for ethological grading towards the animal at different times after the operation respectively. The rat selected by the present invention is a grown male SD rat with low blood vessel aberrance rate, which improves the success rate of manufacturing the model. The bleeding quantity and the bleeding time of the established animal model can be controlled manually, and the operation wound is little, which does not influence the rat limb function and the ethological grading is exact, which has good stability and repeatability.

Description

[0001] The invention relates to a method for establishing an animal model of rat hemorrhagic cerebral infarction, which is used for simulating, researching, preventing and treating human hemorrhagic cerebral infarction and other medical fields. Background technique [0002] Cerebrovascular disease is a disease with high morbidity, high mortality and high disability rate, which seriously affects people's health and quality of life. Cerebrovascular disease ranks second in the statistics of the causes of death among Chinese nationals, accounting for about 14.85%. About 1.5 million patients die of cerebrovascular disease every year, and about 2 / 3 of the survivors are left with disabilities. About 70% of cerebrovascular diseases are ischemic stroke, and hemorrhagic cerebral infarction is a common complication of acute ischemic stroke, and the incidence of hemorrhagic cerebral infarction is reported at home and abroad to be about 3% to 43%. The incidence of hemorrhage after massive ...

Claims

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Application Information

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IPC IPC(8): A61B19/00A61B90/00A61D1/00A61D7/00
Inventor 张祥建刘莹杨晨辉范宏光李俐涛杨燚尹静刘瑞春
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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