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N15/14 isotopic abundance sample collection method and apparatus for determining plant discharging N2O

A technology of isotope abundance and sample collection, which is applied in the direction of measuring devices, sampling devices, instruments, etc., can solve the problems of small emissions, inability to measure samples with stable isotope ratio mass spectrometers, and inability to determine the δ value of plant sources, etc.

Inactive Publication Date: 2008-07-09
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

while plant N 2 O emissions are often small and, as a result, the obtained samples are often not detectable by stable isotope ratio mass spectrometry
[0007] For the reasons mentioned above, it is not possible to measure plant-derived N 2 δ value of O

Method used

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  • N15/14 isotopic abundance sample collection method and apparatus for determining plant discharging N2O

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Including a part 1 with variable shape and volume during the pumping process and a gas collector 2, the plant to be tested 3 is accommodated in the airtight part 1; a gas sampling port 3 is opened on the part 1, and the gas collector 2 passes the gas The sampling port 3 is in sealing connection with the component 1 . The component 1 is provided with a sealing port 4, through which the plant 3 to be measured is accommodated in the component 1, and the sealing port 4 is sealed in the state of gas collection. The part 1 can be a transparent plastic bag or a polytetrafluoroethylene bag. The gas collector 2 can be a needle tube.

[0023] The present invention selects soybean as research object, measures the N of soybean discharge in soybean growing season. 2 δ of O 15 N value.

[0024] Measuring Plant Emissions 15 / 14 N 2 The method of O isotope abundance: in the growing season of soybeans, collect fresh samples of soybean plants respectively as plant samples to be meas...

Embodiment 2

[0030] The difference from Example 1 is:

[0031] The component 1 can be a polytetrafluoroethylene bag; the gas collector 2 can be a gas collection bag. Said use does not contain N 2 O The artificial air is replaced 4-5 times through the sample collection port opened on the measuring device.

[0032] which does not contain N 2 O artificial air is: use high-purity oxygen, nitrogen and CO 2 The volume percentage of gas preparation is oxygen (25%), nitrogen (74%), carbon dioxide (330ppm) without N 2 O's artificial air.

Embodiment 3

[0034] The difference from Example 1 is:

[0035] Said use does not contain N 2 O The artificial air is replaced 6 times through the sample collection port opened on the measuring device. which does not contain N 2 O artificial air is: use high-purity oxygen, nitrogen and CO 2 Gas preparation volume percentage is oxygen (15%), nitrogen (84%) carbon dioxide (390ppm) without N 2 O's artificial air.

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Abstract

The invention relates to the field of stable isotope analysis technology of trace gas, in particular to a sample collection method and a collection device for measuring abundance of <15 / 14>N isotope of N2O discharged by plant. The method comprises the following steps of: disposing a plant sample to be measured in a gas collection device, filling artificial air free of N2O from a collection hole provided on the collection device to exchange air, collecting the gas sample in the device in 20 to 60 min after closing the sample collecting hole, and measuring the value of delta<15>N of N2O in the gas sample by a stable isotope ratio mass spectrometer. The gas collection device comprises a component with variable shape and volume during air pumping process and a gas collector, wherein the plant sample to be detected is accommodated in the sealed component; the gas sampling hole is provided on the component; and a gas collector is hermitically connected with the component through the gas sampling hole. The inventive collection device and the measuring method can conveniently and accurately measure the abundance of stable isotope <15 / 14>N of N2O discharged by a plant system.

Description

technical field [0001] The invention relates to the technical field of stable isotope analysis of trace gases, in particular to a method for measuring N emission from plants. 2 O's 15 / 14 A sample collection method and device for N isotope abundance. Background technique [0002] The main cause of global warming is the increase in the concentration of greenhouse gases in the atmosphere. N 2 O is the third important greenhouse gas, and its single-molecule warming potential is CO 2 The contribution to global warming accounts for about 7% of the total contribution of all greenhouse gases. It also participates in the destruction of stratospheric ozone and enhances the ultraviolet radiation on the surface. Atmospheric N 2 The concentration of O is about 310ppbv with an annual growth rate of 0.25%, which means that the current N 2 The increase of O concentration will have a long-term impact on global climate change. [0003] To the soil-atmosphere interface N 2 The research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/22G01N30/02G01N27/64
Inventor 徐慧张颖李慧倪志龙史荣久杨伟超韩斯琴陈冠雄
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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