Full-automatic concentration determination apparatus based on surface plasma resonance technology

Abstract

The invention discloses a full-automatic concentration measurement device based on surface plasmon resonance technology, which is composed of a liquid processing system, a SPR sensor, a signal detection and controller and a computer. The liquid processing system comprises a sample cell, a multiposition valve, a micro-flow cell, an injection pump, a waste liquid tank and a pump valve controller. The SPR sensor is a miniature non-scanning integrated SPR sensor; the signal detection and controller comprises a control chip C8051F020, a sensor control circuit, a signal processing circuit, an RS232 serial interface and a USB interface circuit and is used for controlling the SPR sensor, receiving SPR light intensity distribution signals of the sensor and transmitting the signals to a computer after simple processing and also for transmitting instructions between the computer and the pump valve controller; and the computer is used for transmitting various control or measurement instructions to the signal detection and controller and calculating the refractive index of a sample by analyzing the SPR light intensity distribution signals, thus obtaining the concentration of the sample to be detected according to a constructed model concerning the sample concentration and the difference in refractive index between the sample and a buffer solution. The invention has the advantages of high generality, high measurement accuracy, less samples, high automation degree, small size and low cost.

Description

Technical field [0001] The invention relates to photoelectric detection technology, microcontroller (MCU) technology, and digital communication technology, in particular to a fully automatic concentration measuring device based on surface plasmon resonance technology. Background technique [0002] Surface plasmon resonance (SPR) is that the evanescent wave generated when light is totally reflected at the interface of glass and metal film causes free electrons on the metal surface to generate surface plasmon (SP), and the surface plasmon and evanescent wave A resonance phenomenon that occurs when the wave frequency and wave number are the same. At this time, the incident light is absorbed, the energy of the reflected light drops suddenly, and a resonance peak (that is, the lowest value of the reflection intensity) appears on the reflection spectrum. The position of the resonance peak and the metal film The refractive index of the medium on the other side is closely related. Accord...

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Problems solved by technology

[0003] At present, the SPR instruments developed at home and abroad are basically large-scale specialized instruments, which have disadvantages such as complex structure, large volume, and high cost, which limit the popularization and application of SPR instruments.

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