Method for regulating, controlling and obtaining sorangium cellulosum liquid ferment bacterium strain by using cell action
A technology of S. cellulosus and liquid fermentation, applied in the direction of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve problems such as no literature or patent reports, achieve good economic benefits, improve efficiency, and replace the times short effect
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Embodiment 1
[0039] This example is to explain how to reduce and remove the exopolysaccharide secretion of S. cellulosus strain, that is, to reduce and remove the exopolysaccharide secretion of S. cellulosus strain by adding non-ionic surfactant in the liquid medium method. Specific steps are as follows:
[0040] 1) Get Sophacystis cellulosus ( Sorangium cellulosum )ATCC25531 dispersed cell suspension was diluted to 5×10 with 20% aqueous glycerol 8 The cells / ml concentration was used as the starting bacterial liquid for UV irradiation. The bacteria liquid is irradiated with a UV mutagenic dose with a lethal rate greater than 95%. During the mutagenesis process, the bacterial solution was stirred slowly with a magnetic stirrer. Coat the MMS plate with the mutagenic bacteria solution, and incubate at 30°C in the dark for 5-7 days. The composition of MMS medium is: soybean peptone 12g / L, potato starch 10g / L, CaCl 2 2H 2 O 1.0g / L, MgSO 4 ·7H 2 O 0.9g / L, Fe-EDTA 7mg / L, agar powder 15g...
Embodiment 2
[0045] This example is to explain how to remove the social cell behavior characteristics of S. cellulosus growth, that is, the method of removing the social cell behavior characteristics of S. cellulosus through serial liquid dilution culture. Specific steps are as follows:
[0046] 1) Get Sophacystis cellulosus ( Sorangium cellulosum ) ATCC15384 was inoculated in 10ml of liquid CDM medium, cultured on a shaker at 30°C and 120 rpm until the early stage of stationary phase. Wherein the composition of CDM culture medium is counted as by weight percentage: skimmed milk powder 0.05%, yeast powder 0.05%, potato starch-1.0%, glycerol 0.2%, CaCl 2 2H 2 O0.15%, MgSO 4 ·7H 2 O 0.15%, Fe-EDTA 10mg / L, HEPES 50mmol / L, pH 7.2.
[0047] 2) Then add 100ml of fresh CDM medium to dilute the bacteria, shake and mix. Take out 10ml of the mixed solution and continue culturing on a shaker at 30°C and 120 rpm for 48 hours.
[0048] 3) Add 100ml of fresh CDM medium to dilute the bacteria, sh...
Embodiment 3
[0053] This example is to explain how to realize the dispersed growth of S. cellulosus in liquid culture, that is, the method of realizing the dispersed growth of S. cellulosus in liquid culture by breaking up cell culture in continuous liquid. Specific steps are as follows:
[0054] 1) Inoculate the solid plate with the cells obtained in Example 2 with loss of growth density dependence for activation, and culture in a humidified incubator at 30°C. The medium components are: soybean peptone 10g / L, potato starch 15g / L, CaCl 2 2H 2 O 1.0g / L, MgSO 4 ·7H 2 O 1.0g / L, Fe-EDTA 10mg / L, agar powder 15g / L.
[0055] 2) Use an inoculation shovel to pick up the expanded bacterial film on the edge and inoculate it on the same solid plate again, roll it apart, and cultivate it until the exponential growth phase.
[0056] 3) Use an inoculation shovel to transfer the bacterium into a 100ml liquid medium shaker flask, crush it on the wall of the flask, and culture it on a shaker at 30°C an...
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