Ribonucleic acid retrogradation snippet compound and applications thereof

A ribonucleic acid and complex technology, which is applied in the field of nucleic acid degradation fragment complexes, can solve the problems of no specific medicine, difficult to cure, etc., and achieve the effect of extensive treatment

Inactive Publication Date: 2008-07-30
周锡漳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Polygenic diseases are diseases caused by a variety of genetic lesions. Common ones include tumors, hypertension, diabetes, AIDS, and aging. These diseases often have no specific medicines and are not easy to cure

Method used

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  • Ribonucleic acid retrogradation snippet compound and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Wash a fetal pig with a body length of about 15 cm, take its liver, kidney and heart, wash away the blood and chop it up, then crush it with a cutting machine, add 0.2M sucrose 10 times its volume, and then add NaCl to The final concentration is 0.14M, treated with a colloid mill, and then centrifuged at a speed of 5000 rpm at 5°C for 20 minutes, and the supernatant obtained is dissolved in 0.01M Tris solution 10 times its volume, and the pH is maintained at 6.0. Add an equal volume of extracting solution, which is a 0.01M Tris solution with pH 6.0 balanced phenol: chloroform: isoamyl alcohol mixed solution in a volume ratio of 1:1:1 / 24, then shake and extract for 10 minutes Centrifuge at 6000 rpm for 20 minutes at 5°C to take the supernatant;

[0054] 2. Extract the supernatant obtained in step 1 with the extract of step 1 above, and centrifuge at 5°C at a speed of 6000 rpm for 20 minutes, and take the supernatant;

[0055] 3. Extract the supernatant obtained in st...

Embodiment 2

[0062] 1. Wash the fetal pig with a body length of about 20 cm, take its liver, kidney and heart, wash away the blood and chop it up, then crush it with a cutting machine, add 0.2M sucrose 10 times its volume, and then add NaCl to The final concentration is 0.14M, treated with a colloid mill, and then centrifuged at a speed of 5000 rpm at 5°C for 20 minutes, and the supernatant obtained is dissolved in 0.01M Tris solution 10 times its volume, and the pH is maintained at 6.0. Add an equal volume of extracting solution, which is a 0.01M Tris solution with pH 6.0 balanced phenol: chloroform: isoamyl alcohol mixed in a volume ratio of 1: 1: 1 / 24, then shake and extract for 15 minutes Then centrifuge at 5°C at a speed of 6000 rpm for 20 minutes to take the supernatant;

[0063] 2. Extract the supernatant obtained in step 1 with the extract of step 1 above, and centrifuge at 5°C at a speed of 6000 rpm for 20 minutes, and take the supernatant;

[0064] 3. Extract the supernatant obt...

Embodiment 4

[0075] Application example

[0076] The ribonucleic acid degradation fragment complex prepared by the embodiment 2 of 1.5ml is dissolved in physiological saline, and the final concentration is 4mg / ml and then the prepared solution is lyophilized by a conventional method to obtain the ribonucleic acid degradation of the present invention Sterile powder for injection of fragment complex.

[0077] For human use, the above-mentioned sterile powder is dissolved in physiological saline according to the dosage standard of 4 mg / kg, and administered through intravenous injection.

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Abstract

The invention relates to a degradation fragment composite of ribonucleic acid, consisting of a plurality of artificial degradation fragments of ribonucleic acid separated and purified from bowels of pigs. The quantity of nucleotide in the artificial degradation fragment of ribonucleic acid ranges from 10 to 300 and the molecular weight ranges from 2*10<2> to 6*10<4>. The invention also relates to the application of the degradation fragment composite of ribonucleic acid in preparation of the medicine for treating polygenic diseases such as liver cancer.

Description

technical field [0001] The invention relates to a nucleic acid degradation fragment complex, in particular to a ribonucleic acid (RNA) degradation fragment complex and application thereof. Background technique [0002] Polygenic diseases are diseases caused by a variety of genetic lesions, such as tumors, hypertension, diabetes, AIDS and aging, etc. These diseases often have no specific medicines and are not easy to cure. [0003] Since 2002, the inhibitory effect of small molecule RNA on various diseases has been gradually discovered and paid attention to. Studies have proved that small molecule RNA has an effective inhibitory effect on tumors, hepatitis C virus, etc., and provides a new way for the treatment of these diseases that plague human beings. [0004] Under the above-mentioned technical background, the present invention applies the latest molecular biology theory and technology, and proposes a ribonucleic acid degradation fragment complex effective for the treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7084A61K31/7088A61K35/23A61K35/34A61K35/407A61P35/00A61P1/16A61K35/22
Inventor 周锡漳
Owner 周锡漳
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