Raw material prescription of opposite phase anion exchange electric chromatograph integral column and preparation thereof
A monolithic column and electrochromatography technology, applied in the field of electrochromatography, can solve the problems of cumbersome and time-consuming steps, and achieve the effect of simple process, good column permeability and less time
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Embodiment 1
[0020] 1. Pretreatment of capillaries
[0021] First rinse the empty capillary column with 0.1mol / L HCl solution for about 30min, then rinse with deionized water for 10min, until the pH is equal to 7.0, rinse with 0.1mol / L NaOH for 3h, then rinse with methanol for 15min, blow dry with nitrogen and set aside . Then add a 1:1 mixture of methanol and methacryloxypropyltrimethoxysilane to the capillary treated in step 1, and react at 60°C for 24h. Then rinse with methanol for 15 min, and blow dry with nitrogen at 25°C.
[0022] 2. In-column synthesis
[0023] Isobutyl methacrylate, N-allyl dimethylamine, ethylene glycol dimethacrylate, N, N-dimethylformamide and 1,4-butanediol are respectively 12.0% by weight , 12.0%, 16.0%, 30.0%, and 30.0% were mixed, and the amount of added initiator azobisisobutyronitrile (AIBN) was 0.3% of the amount of polymer monomer. After the mixture was ultrasonically oscillated for 15 minutes, the reaction solution was Inject into a capillary of a c...
Embodiment 2
[0025] Isobutyl methacrylate, N-allyl dimethylamine, ethylene glycol dimethacrylate, N, N-dimethylformamide and 1,4-butanediol are respectively 10.0% by weight , 10.0%, 20.0%, 30.0%, and 30.0% were mixed, and the amount of added initiator azobisisobutyronitrile (AIBN) was 0.3% of the amount of polymer monomer. After the mixture was ultrasonically oscillated for 15 minutes, the reaction liquid Inject into a capillary of a certain length that has been treated according to step 1, seal both ends of the capillary, and immerse in a water bath at 60°C for 8 hours of reaction. After the reaction is complete, the column is rinsed with methanol to remove possible residual reagents in the capillary.
Embodiment 3
[0027] Isobutyl methacrylate, N-allyl dimethylamine, ethylene glycol dimethacrylate, N, N-dimethylformamide and 1,4-butanediol are respectively 10.0% by weight , 10.0%, 20.0%, 20.0%, and 40.0% were mixed, and the amount of added initiator azobisisobutyronitrile (AIBN) was 0.3% of the amount of polymer monomer. After the mixture was ultrasonically oscillated for 15 minutes, the reaction solution was Inject into a capillary of a certain length that has been treated according to step 1, seal both ends of the capillary, and immerse in a water bath at 60°C for 8 hours of reaction. After the reaction is complete, the column is rinsed with methanol to remove possible residual reagents in the capillary.
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