55 results about "Electro-osmosis" patented technology

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

To provide an electric syringe that realizes stable driving of an electroosmotic flow pump.SOLUTION: An electric syringe comprises: a suction side flow passage part having a flow passage in which driving liquid is stored; and a driving mechanism part having a driving flow passage communicating with the flow passage of the suction side flow passage part. The driving mechanism part includes a pair of electrodes and a porous body sandwiched between the pair of electrodes, and functions as an electroosmotic flow pump. The electric syringe has a tubular relief pipe part whose one end part is connected to a portion between the suction side flow passage part in the driving mechanism part and the porous body.SELECTED DRAWING: Figure 1

The present application relates to a controllable preparation method of a colloidal crystal capillary column and its application in biomolecule separation, and specifically comprises the following steps: a plunger and an electroosmotic pump are prepared in situ at one end of a capillary; a buffer solution containing monodisperse colloidal particles is filled in an upper reservoir of an electric field assembly device, and a buffer solution is filled in a lower reservoir; the capillary is filled with the buffer solution, and the end of the capillary containing the electroosmotic pump is inserted into the lower reservoir; the dispersion of the colloidal solution is maintained by applying oscillation to the upper reservoir, an electric field is applied between the two reservoirs to make the colloidal particles move to the electroosmotic pump under the action of an electroosmotic flow, and a three-dimensional colloidal crystal is assembled; and the capillary is supplemented with an ultrasonic force to reduce lattice defects. The present application has the advantages of simple preparation process, controlled directional movement of colloidal particles through an electric field, improved assembly efficiency and quality of the three-dimensional colloidal crystal, and controllable assembly of the colloidal particles under the action of an electric field through regulation and control of the length and surface charge of the electroosmotic pump.

The present disclosure provides systems and methods for accelerating the aging and / or drying process of making aerogels via the use of an external electric field to induce an electro-osmotic flow inside the pores of an alcogel, rather the relying solely on diffusion-limited mass exchange. To overcome the time and cost of conventional aerogel manufacturing that requires long, involved, and costly processing, the present disclosure provides systems and methods for generating a potential difference across the alcogel to induce advection inside the pores.

This invention belongs to the field of microfluidics, and particularly relates to the use of a pine pollen microrobot combined with a microfluidic chip for cell capture. The pine pollen microrobot is gold-sputtered pine pollen, and the microfluidic chip is injected with the pine pollen microrobot and a conductive cell solution. When an alternating current signal is applied to the microfluidic chip, the fluid surrounding the pine pollen microrobot generates an induced charge electroosmotic vortex, causing cells in the cell solution to move toward the cavity of the pine pollen microrobot, thereby achieving cell capture. Using induced charge electroosmosis (ICEO) technology, this invention enables the efficient capture of blood cells and other micron-sized cell structures. This solves the problem of traditional capture techniques relying on antibody labeling and mechanical capture, reduces operational complexity and capture difficulty, and improves capture success rate and raw material utilization.

The invention discloses a low-voltage polarizable magnetic dielectric microsphere, a preparation method thereof and application of the low-voltage polarizable magnetic dielectric microsphere to adsorption of PFASs, and relates to the technical field of environmental functional materials. The microsphere has a core-shell structure of a conductive magnetic core-dielectric shell, the core is composed of ferroferric oxide and a carbon material, and the shell is a sodium alginate gel layer. The preparation method comprises the following steps: calcining the iron-carbon composite material, mixing a sodium alginate solution, cross-linking calcium lactate / calcium chloride step by step and the like. The microspheres can be subjected to interface polarization under the external 0.2-2 V / cm low-voltage direct-current electric field, a strong local electric field is generated to enhance electrostatic adsorption, meanwhile, the mass transfer and adsorption process of PFASs is synergistically enhanced through electrophoresis and electroosmotic flow effects, the adsorption rate and capacity are remarkably improved, and rapid separation can be achieved through a magnetic field. The material and the method are suitable for efficiently and deeply removing anionic PFASs in wastewater, and have the advantages of low energy consumption, high efficiency, simplicity and convenience in operation and the like.

The present invention relates to the technical field of soil remediation, in particular to a technical method for controlling electroosmotic flow in electrokinetic remediation. The steps are as follows: (1) Test the zeta potential of the target soil under the original pH condition and judge the direction of electroosmotic flow; (2) Install an electrokinetic remediation device and cathode and anode liquid circulation pumps; (3) Adjust the flow rates of the cathode and anode circulation pumps according to the positive and negative values of the soil zeta potential. The present invention solves the technical problem that it is difficult to control the direction and magnitude of electroosmotic flow during electrokinetic remediation, thereby improving the electrokinetic remediation efficiency of heavy metal contaminated soil.

The invention provides a method and a device for dehydration consolidation and directional migration of sludge containing heavy metals, belongs to the technical field of electric consolidation and heavy metal migration, and solves the problems of low sludge dehydration efficiency, unknown heavy metal migration mechanism and poor removal effect in the prior art. The method comprises the following steps: filling a reaction device with sludge containing heavy metals; graphite electrode plates are arranged at the two ends, and filter paper and a permeable stone isolation layer are laid between the electrodes and the sludge; and applying 0.5-2.0 V / cm direct-current voltage gradient for 12-72 hours, maintaining the environment temperature of 25 + / -2 DEG C, driving pore water to migrate to a cathode through electroosmotic flow, dehydrating and solidifying, and meanwhile, enabling heavy metal cations to migrate directionally by utilizing an electrophoresis effect. The matching device comprises an organic glass box body, an adjustable graphite electrode and a current detection and temperature control system. The sludge dewatering efficiency is effectively improved, the method has wide application prospects in the fields of river sludge treatment, polluted site remediation and the like, and a new technical approach is provided for solving the problem of heavy metal polluted sludge treatment.

The invention discloses an optimization method for an electroosmosis drying model of a composite adsorption regenerated material. The optimization method comprises the following steps: acquiring parameters such as electric field intensity, electrokinetic potential, composite material type and proportion, composite material granularity and particle size, composite material particle density and composite material porosity; establishing an initial electroosmosis drying model of the composite adsorption regenerated material according to the parameters; performing a multi-factor coupling simulation test on the initial electroosmosis drying model to obtain a simulation data set containing an electroosmosis flow rate; and comparing the electroosmotic flow rate in the simulation data set with a preset performance standard, and performing iterative optimization on the initial electroosmotic drying model based on a comparison result to obtain an optimized drying model. Core parameters are efficiently screened through sensitivity analysis, rapid iterative optimization of the model is achieved, the output instructive parameters can provide a reliable basis for material selection and design of an electroosmosis dehumidification device, and the performance and practicability of an electroosmosis drying system are guaranteed.

The application discloses a kind of on-line double-gradient pre-concentration detection methods of soil heavy metal ions based on microchip electrophoresis non-contact conductivity detection, including preparation of the analyte sample to be measured, preparation of high concentration buffer as background buffer, preparation of microchip, microchip electrophoresis process and the like steps.When testing, the background buffer contains 2-(N-morpholinyl)ethanesulfonic acid, L-histidine and electroosmotic flow inhibitor;The analyte sample to be measured contains sample matrix liquid and sample extraction stock solution.Among them, the sample matrix liquid is low-concentration buffer, and glacial acetic acid is used to reduce pH value.In testing, due to the existence of concentration gradient and pH gradient between the analyte sample solution to be measured and the background buffer, the ions to be measured in the sample will slow down and accumulate at the contact surface of the two during migration, realizing on-line pre-concentration, and improving the detection sensitivity of the ions to be measured.The application greatly improves the separation degree of multiple heavy metal ions, and effectively improves the detection sensitivity of heavy metal ions.

The present invention provides a system and method for capturing polynucleotide chains on a defined channel surface. [Solution] The method may include the steps of providing a device having a channel, and providing a sample containing a polymer capable of altering the electroosmotic flow within the channel and a polynucleotide chain to a defined surface of the channel. The method may also include the steps of physically interacting the polymer with a defined surface of the channel, and applying an electric field to the defined surface of the channel to promote physical interaction between the polynucleotide chain and the polymer and to trap the polynucleotide chain on the defined surface of the channel.

The microfluidic device comprises a shell, a micro-column screening area, a positive electrode, a negative electrode and a cover plate, the positive electrode and the negative electrode are installed on the left side and the right side of the interior of the shell, the micro-column screening area is arranged between the positive electrode and the negative electrode, and the shell is covered with the cover plate; the micro-column screening area is composed of a micro-column array, and micro-channels for sperms to pass through are formed among micro-columns. The invention further discloses a manufacturing method of the micro-column array. The rapid sperm screening device is simple to operate. According to the invention, a micro-nano processing means is adopted to prepare a micro-channel environment for simulating sperm swimming of a microstructure, a high-viscosity buffer solution is adopted to simulate a body fluid environment for sperm swimming, and an electroosmotic flow method is adopted, so that a liquid sample moves from a positive electrode direction to a negative electrode direction, a sperm flowing environment is created in situ, and active sperm screening is accelerated.

The present application discloses a heat exchange regulation system for a battery pack, comprising a microchannel heat exchanger, a plurality of interdigital electrodes, a swinging assembly, and a local early warning module. The microchannel heat exchanger is provided with a flow channel, and the local early warning module induces electroosmosis through the interdigital electrodes, thereby removing bubbles near the interdigital electrodes. The swinging assembly comprises a first magnetic component and a plurality of swinging components. The local early warning module controls the intermittent power supply of the first magnetic component to drive the swinging component to swing, thereby shearing and peeling off bubbles attached to the wall of the flow channel. By superimposing the mainstream of the electroosmotic flow and the swinging eddy current, the bubbles in the flow channel are reduced, and the local thermal resistance is significantly improved. The local early warning module adopts the coupling control of the DRL adaptation layer and the LAMPC execution layer to achieve real-time defoaming and optimize the flow and heat exchange performance. Through the closed-loop control strategy of coordinated sensing of temperature and pressure, coordinated defoaming of electroosmotic flow and mechanical disturbance, intelligent early warning, and dynamic feedback optimization, efficient and stable operation of microchannel heat exchange under high heat flux density is achieved.

Systems and methods for trapping a polynucleotide strand at a defined surface of a channel. Methods may include providing a device including a channel and providing to a defined surface of the channel a polymer capable of modifying an electroosmotic flow in the channel and a sample including a polynucleotide strand. Methods may also include physically interacting the polymer with the defined surface of the channel and applying an electric field to the defined surface of the channel to promote a physical interaction between the polynucleotide strand and the polymer to trap the polynucleotide strand at the defined surface of the channel.

The present invention relates to a method for purifying labeled glycans by electroosmotic flow assisted capillary zone electrophoresis, or a method for on-line electrokinetic purification of a labeled reaction mixture of labeled glycans and free labeling agent, wherein the method is performed in a separation mode, wherein the free labeling agent and the labeled glycans migrate in opposite directions, thereby separating the free labeling agent from the glycans. The method according to the present invention allows for a purification-free capillary zone electrophoresis analysis of labeled glycans. Furthermore, the method allows for separation in a shorter analysis time without the need for a sample purification step.

The application discloses a numerical simulation method of an electrically assisted microbial remediation process, and belongs to a numerical simulation method system. First, a conceptual model, chemical substance setting, initial condition and boundary condition of a research area are determined through initialization research area setting. Then, the number of simulation nodes is set based on grid self-adaptive division. A migration model established by Nernst-Planck-Poisson equation is considered in COMSOL software based on a migration-reaction operator separation method, so as to describe processes such as convection, dispersion, electromigration and electroosmotic flow related to material migration in groundwater. In PHREEQC, the influence of microbial growth on the permeability coefficient and the multivariate inhibition Monod degradation reaction are considered. Finally, the CPM-BIO numerical simulation method is formed by combination, so that the electrically assisted microbial remediation process can be simulated in detail. Compared with existing calculation models, the CPM-BIO can realize the detailed description of the electrically assisted microbial remediation numerical simulation process with multiple dimensions and multiple reactions.

The utility model relates to the field of bipolar microfluidics, and discloses a bipolar microfluidics production device which comprises a bearing table, a microfluidics main body is fixedly arranged on the upper surface of the bearing table, a chip is fixedly arranged on the upper surface of the bearing table, and a blue light tower is fixedly arranged on the upper surface of the bearing table. According to the device, through the arrangement of the micro-fluidic main body, primary fluid treatment can be carried out on liquid, through the arrangement of the chip, high-precision control can be carried out on small particles and cells in the fluid through the synergistic effect of an electric field generated by the bipolar electrode, electroosmotic flow and dielectrophoretic force, and through the arrangement of the heat preservation sleeve and the temperature adjusting instrument, high-precision control can be carried out on the small particles and cells in the fluid. The temperature of the fluid in transmission can be accurately controlled, the fluid can be specifically treated through the arrangement of a blue light tower, the activity of a biological sample and the stability of chemical substances in the fluid can be effectively protected through the arrangement of a low-temperature collector, and the fluid can be uniformly treated through the arrangement of a blue light emission vessel and a rotor.

The application relates to a capillary electrophoresis system and method combined with a non-continuous sample injection mass spectrum, wherein the system comprises: an electrophoretic separation-electrospray ionization module for separating components in a target analyte according to different electrophoretic mobilities; a real-time adjustable high-voltage power supply device for providing a voltage required by capillary electrophoresis according to the requirements of an electroosmotic flow and an electrophoretic speed in a capillary; a target component signal feedback module for detecting a target analyte or a real-time electroosmotic flow speed; an electrophoretic current measurement and migration time correction module; a real-time feedback signal processing and control module; and a non-continuous sample injection mass spectrum system. Thus, the problems in the prior art that, when a continuous capillary electrophoresis technology is combined with a non-continuous sample injection small mass spectrum system, the accuracy is reduced, the analysis quality is poor, the sampling frequency is greatly limited, it is difficult to ensure that mass spectrum data at the highest point of a chromatographic peak can be collected, and the sensitivity of detection and the accuracy of quantitative analysis are affected are solved.

The invention discloses application technical equipment for enhancing oil extraction, water shutoff, oil increase and recovery efficiency improvement through electroosmotic flow, belongs to the technical field of oil exploitation, and solves the problems that an existing device only depends on basic electroosmotic flow to act on an oil reservoir, dead oil areas and remaining oil areas are difficult to activate, and the yield increasing effect is limited. Comprising a combined transformer, a switch cabinet, a high-frequency oil extraction power supply device, a transmission cable and a sleeve group, according to the device, the high-frequency oil extraction power source device is arranged and can accurately dredge a dead oil area, a remaining oil area and a capillary bound water area which are not used in water injection development through the synergistic effect of multiple mechanisms of fracture forming, water plugging and electrochemical plugging removal of high-frequency pulse electromagnetic waves, the swept volume is increased, meanwhile, a water film on the surface of rock is driven to be thinned, and the oil extraction efficiency is improved. A seepage space is vacated for oil flow, the water reducing and oil increasing effects are more remarkable, and the purpose of high recovery efficiency is achieved.

The present invention discloses a structurally stable and easily injectable hydroxyapatite microsphere and a preparation method thereof, specifically relating to the field of biomedical materials technology. The preparation method comprises constructing a microfluidic reaction system, utilizing electroosmotic flow to precisely control the flow rate of the solution, mixing and adjusting the pH value, applying a pulsed electric field, reacting in a microfluidic chip, filtering through an ultrafiltration membrane, washing with supercritical carbon dioxide fluid extraction, freeze drying, and airflow milling, and finally mixing with a biodegradable polymer solution to prepare the microspheres by electrostatic spraying technology. The prepared hydroxyapatite microspheres are surface-grafted with arginine-glycine-aspartic acid polypeptides, with a particle size of 20-35 μm, a particle size distribution coefficient of variation of less than 8%, a compressive strength of 5-8 MPa, and a mass loss rate of less than 5% after immersion in a simulated physiological environment solution for 30 days. The microspheres have the advantages of stable structure, easy injection, and good biocompatibility, and have good application prospects in the fields of biomedicine and the like.

A drug liquid transport device (1) according to the present invention comprises: a drive liquid accommodation unit (11) that accommodates a drive liquid (13); a transport liquid accommodation unit (12) that accommodates a drug liquid (18); an electroosmotic flow pump (14) that transports the drive liquid between the drive liquid accommodation unit and the transport liquid accommodation unit; and a needle for injecting the drug liquid supplied from the transport liquid accommodation unit (12) into the skin. The transport liquid accommodation unit (12) comprises: a first chamber that accommodates the drug liquid and that is connected to a needle hole of the needle; and a second chamber that accommodates the drive liquid and is separated from the first chamber by a diaphragm. The drug liquid transport device is configured such that the electroosmotic flow pump transports the drive liquid from the drive liquid accommodation unit to the second chamber to change the capacity of the first chamber, whereby the drug liquid (18) is injected into the skin.

This invention provides a microcurrent rapid drying system and application method for metallic materials, belonging to the field of metallic material processing technology. It aims to solve the technical problems of low drying efficiency, easy oxidation, and uneven drying in traditional metallic materials drying processes. The system includes a chamber with a multi-layer material conveying mechanism. The conveying mechanism includes an insulated shaft drive assembly and a metal screen conveyor belt rotating in the opposite direction outside the chamber. The inner wall of the chamber is equipped with staggered insulating baffles to guide the material to tumble and fall. The system also includes an electrode module, which uses an external power supply and a constant-pressure spring-driven brush slidably connected to the metal screen conveyor belt to provide a microcurrent to the metallic material. An auxiliary module and a gas-liquid treatment module are connected to the outer side of the chamber for material feeding and discharging, drainage, and closed-loop circulation of inert gas. This invention utilizes electroosmosis and Joule heating to synergistically enhance drying, avoiding high-temperature oxidation and achieving rapid, non-destructive, and uniform drying at low or room temperature, significantly reducing drying energy consumption.

The invention relates to the technical field of capillary electrophoresis separation, and discloses a preparation method and application of an LP (HPMA) coating capillary, and the preparation method comprises the following steps: step 1, filling a chain transfer reagent (CTA) cyanomethyl [3-(trimethoxysilyl) propyl] trithiocarbonate into a capillary subjected to acid and alkali treatment through inert gas, sealing two ends of the capillary, and performing vacuum drying; performing water bath incubation; after incubation, residual reagents are removed, and the CTA modified capillary tube is obtained; 2, N-(2-hydroxypropyl) methacrylamide and 4, 4 '-azobis (4-cyano valeric acid) are mixed in a sodium acetate buffer solution at the room temperature, and a uniform pre-polymerization solution is formed; and 3, introducing the pre-polymerized solution obtained in the step 2 into the CTA modified capillary tube obtained in the step 1, carrying out water bath incubation, and removing the residual reagent after incubation. The LP (HPMA) coating capillary tube is good in biocompatibility and pH stability, low in electroosmotic flow (EOF) and good in proteomics sample separation effect.

This invention discloses a highly sensitive chondrocyte cytoplasmic viscosity probe and its preparation method, belonging to the field of cell probe technology. The method includes: drawing a borosilicate capillary into a nanoparticle with an inner diameter of 70-490 nm; filling the tube lumen with an aminosilane solution and incubating at room temperature to achieve amino functionalization modification; and finally injecting PBS buffer to obtain the probe. Amino modification significantly enhances the charge density of the nanoparticle's inner wall. When the inner diameter of the nanoparticle tip is reduced to 120 nm, the spatial confinement effect significantly enhances the regulatory effect of the enhanced surface charge on ion transport, strengthens the electric double layer effect and electroosmotic effect, and makes the ion current highly sensitive to changes in solution viscosity, thereby greatly improving the probe's response sensitivity to small viscosity fluctuations. The preparation method of this invention is simple and efficient, and the resulting probe has a fast response speed, enabling real-time dynamic monitoring of chondrocyte cytoplasmic viscosity, providing a highly sensitive tool for early warning and mechanism research of diseases such as osteoarthritis.

The invention discloses a hydroxyapatite microsphere stable in structure and easy to inject and a preparation method thereof, and particularly relates to the technical field of biomedical materials.According to the preparation method, a microfluidic reaction system is constructed, the flow velocity of a solution is accurately controlled through electroosmotic flow, the solution is mixed, the pH value is adjusted, a reaction is conducted in a microfluidic chip after a pulsed electric field is applied, and the hydroxyapatite microsphere with the stable structure and the easy to inject is obtained. Filtering by an ultrafiltration membrane, extracting and washing by a supercritical carbon dioxide fluid, freeze-drying, crushing by an airflow mill, finally mixing with a biodegradable polymer solution, and preparing the microspheres by an electrostatic spraying technology. The arginine-glycine-aspartic acid polypeptide is grafted on the surface of the prepared hydroxyapatite microsphere, the particle size is 20-35 microns, the particle size distribution variable coefficient is smaller than 8%, the compressive strength reaches 5-8 MPa, the mass loss rate is smaller than 5% after the hydroxyapatite microsphere is soaked in a simulated physiological environment solution for 30 days, and the hydroxyapatite microsphere has the advantages of being stable in structure, easy to inject, good in biocompatibility and the like. Good application prospects are realized in the fields of biomedicine and the like.

The invention provides a method for detecting the content of boric acid based on capillary electrophoresis, which is used for analyzing and detecting the content of boric acid in a boric acid sample solution by utilizing the advantages of electrophoresis mobility difference, influence of electroosmotic flow, interaction among separation media and the like of a capillary electrophoresis technology. And the influence of a quality discrimination effect and a memory effect can be effectively eliminated.

The invention relates to the technical field of water pollution treatment, in particular to a method for restoring a river water body by utilizing high-energy potential, which comprises the following steps: S1, generating an external electric field, and establishing an electrophoresis and electroosmotic flow field from outside to inside in the water body around a restoration unit to drive the water body and pollutants to migrate into the restoration unit; s2, performing in-cavity degradation, and performing decomposition treatment on the water body and pollutants migrated into the synergistic reaction cavity in the remediation unit through an oxidation process; s3, state sensing is carried out, the electrical impedance of the water body in the internal collaborative reaction cavity is measured in real time, and a real-time impedance value representing the pollutant treatment load in the cavity is obtained. According to the invention, the water body impedance of the internal reaction cavity is used as a real-time characterization parameter of the pollutant treatment load, and the capture rate of external pollutants and the degradation rate of internal pollutants are dynamically balanced, so that the remediation system can automatically adapt to the dynamic change of river water quality, and the operation stability is improved.

The invention discloses a method for efficiently removing heavy metal ions in livestock and poultry manure, which adopts an eluting agent to form a stable chelate with the heavy metal ions through a coordination effect, realizes efficient total removal of the chelate from a manure solid phase, and breaks through the limitation that only the bioavailability can be reduced by a traditional passivation technology. The manure solid phase is subjected to neutral adjustment and then mixed with straw, a compound microbial agent composed of cellulose decomposition bacteria, nitrogen-fixing bacteria and phosphate solubilizing bacteria is inoculated, aerobic fermentation is carried out, and the high-quality organic fertilizer is prepared; leacheate containing heavy metal is subjected to electric-PRB combined treatment, the heavy metal is promoted to be enriched on a PRB material by utilizing electroosmotic flow and electrophoretic migration, and then phosphorus resources are recovered by a struvite crystallization process. According to the method, the removal rate of copper in the livestock and poultry manure is 96.2%, the removal rate of zinc in the livestock and poultry manure is 91.3%, the removal rate of cadmium in the livestock and poultry manure is 98.0%, the agricultural-grade organic fertilizer and the phosphorus recovery liquid are obtained, the process is flexible, the cost is low, industrialization popularization is easy, and the method is suitable for manure treatment of different livestock and poultry types and pollution levels.

The application relates to a small extracellular vesicle detection method based on a solid-state nanopore and machine learning. The method comprises the following steps: detecting a to-be-detected sample by using a solid-state nanopore chip, making an electroosmotic flow dominate particle capture and a through hole by applying a voltage; collecting a nanopore ion current signal and performing filtering, baseline correction and detrending pretreatment; extracting equivalent charge loss, blocking amplitude, half-residence time, left edge slope and right edge slope of a single event through hole signal; inputting the features into a trained machine learning model to obtain a specific event correlation score, taking a correlation event ratio in a sample as a discrimination index, and realizing detection of target small extracellular vesicles. Compared with an existing detection method which needs labeling and enrichment, the application can realize rapid, label-free and single-particle-level detection analysis in a complex plasma matrix.

The application provides an electric response biomimetic flow guide composite material and a preparation method and application thereof, and a sludge treatment method, and belongs to the technical field of solid waste treatment and water environment treatment. The electric response biomimetic flow guide composite material comprises a porous biomimetic matrix and a polypyrrole conductive layer covering the surface and the pore channel of the porous biomimetic matrix; and a metal adsorption layer loaded on the surface of the polypyrrole conductive layer or the porous biomimetic matrix; and the porous biomimetic matrix has a through capillary channel in the longitudinal direction. The polypyrrole conductive layer and the metal adsorption layer are constructed on the surface and the pore channel of the porous biomimetic matrix, under the action of an external electric field, the synergistic effect of capillary action, electroosmosis and electromigration is realized, the water in the sludge is migrated along the flow guide direction, and the pollutants such as heavy metals are enriched in a specific area, so that the spatial separation of water and pollutants is realized.