Micro flow control chip capillary electrophoresis negative pressure sampling method

A technology of microfluidic chip and capillary electrophoresis, applied in separation methods, chemical instruments and methods, material analysis through electromagnetic means, etc., can solve the problems of infusion equipment with high voltage and affect safe operation, and avoid discrimination effect , improved safety, and improved sample injection speed
CN1737562AInactive Publication Date: 2006-02-22ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2006-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to one electrophoresis sample in and isolation method of micro flow control capillary, which is characterized by the following: using micro flow control chip network structure; adopting column plug pump and taking sample from waste pool air through three-way valve and interface to form negative pressure; the micro flow chip and other sample solution and buffer liquid flowing downward and forming stable sample plug in the crossing place of sample in channel and isolation channel and connecting the valve with air through three-way valve.
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Description

Technical field

[0001] The invention relates to a microfluidic chip capillary electrophoresis analysis technology, in particular to a negative pressure sampling and separation technology on a microfluidic chip capillary electrophoresis. Background technique

[0002] Since the concept of micro-total analysis system was proposed in 1990, microfluidic chip technology has opened up a broad space for development in the fields of medicine and life sciences. Microfluidic chip capillary electrophoresis technology has been used in DNA sequencing, separation and identification of DNA fragments, separation and determination of amino acids, peptides, proteins, and analysis of components in single cells.

[0003] For the above applications, accurate control of the picoliter sample injection volume is the key to separation and determination. At present, electric sampling methods based on electroosmotic flow drive are widely used, such as electro-clamping sampling, suspension sampling and...

Claims

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